Laboratory diagnosis of neurocysticercosis (taenia solium)

The Cysticercosis Working Group in Peru

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Neurocysticercosis accounts for approximately 30% of all epilepsy cases in most developing countries. The immunodiagnosis of cysticercosis is complex and strongly influenced by the course of infection, the disease burden, the cyst location, and the immune response of the host. The main approach to immunodiagnosis should thus be to evaluate whether the serological results are consistent with the diagnosis suggested by imaging. Antibody detection is performed using lentil lectin-purified parasite antigens in an enzyme-linked immunoelectrotransfer blot format, while antigen detection uses a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA). Promising new assay configurations have been developed for the detection of both antibody and antigen, including assays based on synthetic or recombinant antigens that may reduce costs and improve assay reproducibility and multiplex bead-based assays that may provide simultaneous quantitative results for several target antigens or antibodies.

Original languageEnglish (US)
Article numbere00424
JournalJournal of Clinical Microbiology
Volume56
Issue number9
DOIs
StatePublished - Sep 1 2018

Fingerprint

Taenia solium
Neurocysticercosis
Clinical Laboratory Techniques
Antigens
Immunologic Tests
Antibodies
Cysticercosis
Developing Countries
Cysts
Epilepsy
Parasites
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Costs and Cost Analysis
Enzymes
Infection

Keywords

  • Antibody
  • Antigen
  • Cysticercosis
  • EITB
  • ELISA
  • Neurocysticercosis
  • Peru
  • Taenia solium
  • Western blot

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Laboratory diagnosis of neurocysticercosis (taenia solium). / The Cysticercosis Working Group in Peru.

In: Journal of Clinical Microbiology, Vol. 56, No. 9, e00424, 01.09.2018.

Research output: Contribution to journalArticle

The Cysticercosis Working Group in Peru. / Laboratory diagnosis of neurocysticercosis (taenia solium). In: Journal of Clinical Microbiology. 2018 ; Vol. 56, No. 9.
@article{7af9f34f02b64399bd60c11ae283b6b6,
title = "Laboratory diagnosis of neurocysticercosis (taenia solium)",
abstract = "Neurocysticercosis accounts for approximately 30{\%} of all epilepsy cases in most developing countries. The immunodiagnosis of cysticercosis is complex and strongly influenced by the course of infection, the disease burden, the cyst location, and the immune response of the host. The main approach to immunodiagnosis should thus be to evaluate whether the serological results are consistent with the diagnosis suggested by imaging. Antibody detection is performed using lentil lectin-purified parasite antigens in an enzyme-linked immunoelectrotransfer blot format, while antigen detection uses a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA). Promising new assay configurations have been developed for the detection of both antibody and antigen, including assays based on synthetic or recombinant antigens that may reduce costs and improve assay reproducibility and multiplex bead-based assays that may provide simultaneous quantitative results for several target antigens or antibodies.",
keywords = "Antibody, Antigen, Cysticercosis, EITB, ELISA, Neurocysticercosis, Peru, Taenia solium, Western blot",
author = "{The Cysticercosis Working Group in Peru} and Garcia, {Hector H.} and Seth O'Neal and John Noh and Sukwan Handali and Gilman, {Robert H.} and Gonzalez, {Armando E.} and Tsang, {Victor C.W.} and Silvia Rodriguez and Manuel Martinez and Isidro Gonzales and Herbert Saavedra and Manuela Verastegui and Bustos, {Javier A.} and Mirko Zimic and Holger Mayta and Yesenia Castillo and Yagahira Castro and Lopez, {Maria T.} and Gavidia, {Cesar M.} and Moyano, {Luz M.} and Ricardo Gamboa and Claudio Muro and Percy Vilchez and Nash, {Theodore E.} and Siddhartha Mahanty and Jon Friedland",
year = "2018",
month = "9",
day = "1",
doi = "10.1128/JCM.00424-18",
language = "English (US)",
volume = "56",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Laboratory diagnosis of neurocysticercosis (taenia solium)

AU - The Cysticercosis Working Group in Peru

AU - Garcia, Hector H.

AU - O'Neal, Seth

AU - Noh, John

AU - Handali, Sukwan

AU - Gilman, Robert H.

AU - Gonzalez, Armando E.

AU - Tsang, Victor C.W.

AU - Rodriguez, Silvia

AU - Martinez, Manuel

AU - Gonzales, Isidro

AU - Saavedra, Herbert

AU - Verastegui, Manuela

AU - Bustos, Javier A.

AU - Zimic, Mirko

AU - Mayta, Holger

AU - Castillo, Yesenia

AU - Castro, Yagahira

AU - Lopez, Maria T.

AU - Gavidia, Cesar M.

AU - Moyano, Luz M.

AU - Gamboa, Ricardo

AU - Muro, Claudio

AU - Vilchez, Percy

AU - Nash, Theodore E.

AU - Mahanty, Siddhartha

AU - Friedland, Jon

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Neurocysticercosis accounts for approximately 30% of all epilepsy cases in most developing countries. The immunodiagnosis of cysticercosis is complex and strongly influenced by the course of infection, the disease burden, the cyst location, and the immune response of the host. The main approach to immunodiagnosis should thus be to evaluate whether the serological results are consistent with the diagnosis suggested by imaging. Antibody detection is performed using lentil lectin-purified parasite antigens in an enzyme-linked immunoelectrotransfer blot format, while antigen detection uses a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA). Promising new assay configurations have been developed for the detection of both antibody and antigen, including assays based on synthetic or recombinant antigens that may reduce costs and improve assay reproducibility and multiplex bead-based assays that may provide simultaneous quantitative results for several target antigens or antibodies.

AB - Neurocysticercosis accounts for approximately 30% of all epilepsy cases in most developing countries. The immunodiagnosis of cysticercosis is complex and strongly influenced by the course of infection, the disease burden, the cyst location, and the immune response of the host. The main approach to immunodiagnosis should thus be to evaluate whether the serological results are consistent with the diagnosis suggested by imaging. Antibody detection is performed using lentil lectin-purified parasite antigens in an enzyme-linked immunoelectrotransfer blot format, while antigen detection uses a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA). Promising new assay configurations have been developed for the detection of both antibody and antigen, including assays based on synthetic or recombinant antigens that may reduce costs and improve assay reproducibility and multiplex bead-based assays that may provide simultaneous quantitative results for several target antigens or antibodies.

KW - Antibody

KW - Antigen

KW - Cysticercosis

KW - EITB

KW - ELISA

KW - Neurocysticercosis

KW - Peru

KW - Taenia solium

KW - Western blot

UR - http://www.scopus.com/inward/record.url?scp=85052492481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052492481&partnerID=8YFLogxK

U2 - 10.1128/JCM.00424-18

DO - 10.1128/JCM.00424-18

M3 - Article

VL - 56

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 9

M1 - e00424

ER -