Label-free in vivo optical imaging of functional microcirculations within meninges and cortex in mice

Yali Jia, Ruikang K. Wang

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Abnormal microcirculation within meninges is common in many neurological diseases. There is a need for an imaging method that is capable of monitoring dynamic meningeal microcirculations, preferably decoupled from cortical blood flow. Optical microangiography (OMAG) is a recently developed label-free imaging method capable of producing 3D images of dynamic blood perfusion within micro-circulatory tissue beds at an imaging depth up to ∼2 mm, with an unprecedented imaging sensitivity to blood flow at ∼4 μm/s. In this paper, we demonstrate the utility of OMAG in imaging the detailed blood flow distributions, at a capillary level resolution, within the meninges and cortex in mice with the cranium left intact. Using a thrombotic mouse model, we show that the OMAG can yield longitudinal measurements of meningeal vascular responses to the insult and can decouple these responses from those in the cortex, giving valuable information regarding the localized hemodynamics along with the dynamic formation of thrombotic event. The results indicate that OMAG can be a useful tool to study therapeutic strategies in preclinical animal models in order to mitigate various pathologies that are mainly related to the meningeal circulations.

Original languageEnglish (US)
Pages (from-to)108-115
Number of pages8
JournalJournal of Neuroscience Methods
Volume194
Issue number1
DOIs
StatePublished - Dec 15 2010

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Keywords

  • Blood flow
  • Meningeal blood vessel network
  • Microcirculation
  • Optical microangiography
  • Thrombosis

ASJC Scopus subject areas

  • Neuroscience(all)

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