L-selectin and leukocyte function in skeletal muscle reperfusion injury

Daniel D. Lozano, Edward (Ed) Kahl, Howard P. Wong, Linda L. Stephenson, William A. Zamboni

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Hypothesis: Treatment with anti-L-selectin monoclonal antibody will reduce venular neutrophil-endothelial rolling (flux and velocity) and adhesion associated with ischemia reperfusion injury in rat skeletal muscle. Design: Prospective, randomized experimental trials. Setting: Basic science research laboratory. Materials: Male Wistar rats weighing 109 ± 5 g (mean ± SEM). Interventions: Gracilis pedicle muscle flaps were elevated and microcirculation was observed by intravital microscopy: Two groups were evaluated: (1) the control group, which received 4 hours of global ischemia, and (2) the experimental group, which received 4 hours of global ischemia, plus treatment with anti-c-selectin monoclonal antibody 30 minutes before reperfusion. Main Outcome Measures: The number of rolling and adherent leukocytes in postcapillary venules were counted in the 2 groups at baseline and at 1 through 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion. Results: Treatment with the monoclonal antibody to L-selectin significantly reduced the number of rolling leukocytes (flux) at 2 through 5, 20, 30, 45, and 60 minutes of reperfusion compared with controls (P <.05). Use of the monoclonal antibody significantly reduced the number of adherent neutrophils at 5, 10, 15, 20, 30, 45, and 60 minutes of reperfusion [P

Original languageEnglish (US)
Pages (from-to)1079-1081
Number of pages3
JournalArchives of Surgery
Volume134
Issue number10
DOIs
StatePublished - Oct 1999
Externally publishedYes

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L-Selectin
Reperfusion Injury
Reperfusion
Skeletal Muscle
Leukocytes
Monoclonal Antibodies
Leukocyte Rolling
Neutrophils
Ischemia
Selectins
Venules
Microcirculation
Leukocyte Count
Wistar Rats
Therapeutics
Outcome Assessment (Health Care)
Control Groups
Research

ASJC Scopus subject areas

  • Surgery

Cite this

Lozano, D. D., Kahl, E. E., Wong, H. P., Stephenson, L. L., & Zamboni, W. A. (1999). L-selectin and leukocyte function in skeletal muscle reperfusion injury. Archives of Surgery, 134(10), 1079-1081. https://doi.org/10.1001/archsurg.134.10.1079

L-selectin and leukocyte function in skeletal muscle reperfusion injury. / Lozano, Daniel D.; Kahl, Edward (Ed); Wong, Howard P.; Stephenson, Linda L.; Zamboni, William A.

In: Archives of Surgery, Vol. 134, No. 10, 10.1999, p. 1079-1081.

Research output: Contribution to journalArticle

Lozano, DD, Kahl, EE, Wong, HP, Stephenson, LL & Zamboni, WA 1999, 'L-selectin and leukocyte function in skeletal muscle reperfusion injury', Archives of Surgery, vol. 134, no. 10, pp. 1079-1081. https://doi.org/10.1001/archsurg.134.10.1079
Lozano, Daniel D. ; Kahl, Edward (Ed) ; Wong, Howard P. ; Stephenson, Linda L. ; Zamboni, William A. / L-selectin and leukocyte function in skeletal muscle reperfusion injury. In: Archives of Surgery. 1999 ; Vol. 134, No. 10. pp. 1079-1081.
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