L-AP4 inhibits calcium currents and synaptic transmission via a G-protein-coupled glutamate receptor

Paul Q. Trombley, Gary Westbrook

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

The AP4 (2-amino-4-phosphonobutyrate) receptor is a presynaptic glutamate receptor that inhibits transmitter release via an unknown mechanism. We examined the action of L-AP4 on voltage-dependent calcium currents and excitatory synaptic transmission on cultured olfactory bulb neurons using whole-cell voltage-clamp methods. In neurons dialyzed with GTP, L-AP4 inhibited high-threshold calcium currents evoked in barium solutions. The inhibition was irreversible in the presence of GTP-γ-S and blocked by removing intracellular Mg2+ or by preincubation with pertussis toxin (PTX), consistent with the involvement of a PTX-sensitive G-protein. Dialysis with staurosporine or buffering of intracellular calcium to pCa

Original languageEnglish (US)
Pages (from-to)2043-2050
Number of pages8
JournalJournal of Neuroscience
Volume12
Issue number6
StatePublished - Jun 1992

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Glutamate Receptors
G-Protein-Coupled Receptors
Synaptic Transmission
Pertussis Toxin
Guanosine Triphosphate
Calcium
Presynaptic Receptors
Neurons
Staurosporine
Olfactory Bulb
Barium
GTP-Binding Proteins
Dialysis
2-amino-4-phosphono-propinate

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

L-AP4 inhibits calcium currents and synaptic transmission via a G-protein-coupled glutamate receptor. / Trombley, Paul Q.; Westbrook, Gary.

In: Journal of Neuroscience, Vol. 12, No. 6, 06.1992, p. 2043-2050.

Research output: Contribution to journalArticle

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