Kinetics of Repair of UV-induced DNA Damage in Repair-proficient and -deficient Cells as Determined by Quantitative Polymerase Chain Reaction

Michael J. McCarthy, Judah I. Rosenblatt, Robert (Stephen) Lloyd

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Advances in methodologies to monitor gene-specific repair in human cells have facilitated a detailed understanding of the complexity of the nucleotide excision repair system. One of these procedures, quantitative polymerase chain reaction (QPCR), holds significant promise for dissecting the fine structure of the repair of UV-induced DNA damage. This assay was used to study the repair of UV photoproducts in both actively transcribed and nontranscribed genes from human cells that were capable of (1) repair of both cyclobutane pyrimidine dimers and 6-4 photoproducts; (2) removal of neither dimers nor 6-4 photoproducts; (3) strong preferential repair of 6-4 photoproducts relative to dimers; and (4) severely depressed rates of 6-4 photoproducts and dimers. Detailed kinetic analyses revealed that repair of both active and inactive genes can be studied with a very fine degree of precision and that the repair status of the cells can easily be detected by use of the procedures described.

Original languageEnglish (US)
Pages (from-to)356-362
Number of pages7
JournalPhotochemistry and Photobiology
Volume66
Issue number3
StatePublished - Sep 1997
Externally publishedYes

Fingerprint

polymerase chain reaction
Polymerase chain reaction
DNA Damage
Repair
deoxyribonucleic acid
dimers
genes
damage
Polymerase Chain Reaction
Kinetics
DNA
kinetics
cells
Genes
Pyrimidine Dimers
Dimers
cyclobutane
DNA Repair
nucleotides
pyrimidines

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Kinetics of Repair of UV-induced DNA Damage in Repair-proficient and -deficient Cells as Determined by Quantitative Polymerase Chain Reaction. / McCarthy, Michael J.; Rosenblatt, Judah I.; Lloyd, Robert (Stephen).

In: Photochemistry and Photobiology, Vol. 66, No. 3, 09.1997, p. 356-362.

Research output: Contribution to journalArticle

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