Kinetics of phosphatidylinositol-3-phosphate acquisition differ between lgG bead-containing phagosomes and Mycobacterium tuberculosis-containing phagosomes

Georgiana Purdy, Róisín M. Owens, Linda Bennett, David G. Russell, Barbara A. Butcher

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A key aspect of Mycobacterium tuberculosis pathogenesis is the ability of the bacteria to survive within the host macrophage. A phagosome containing an IgG-coated bead matures into a lysosomal compartment as evidenced by a decrease in pH and an increased acquisition of hydrolytic enzymes. In contrast, when M. tuberculosis is phagocytosed, the maturation of the bacteria-containing phagosome is arrested, and the bacterium resides within a vacuole that retains characteristics of early endosomal compartments. M. tuberculosis-containing phagosomes are delayed in the recruitment of the early endosome autoantigen EEA1. Acquisition of EEA1 is dependent on the presence of phosphatidylinositol-3-phosphate (PI-3-P) generated by the kinase Vps34. We tested the hypothesis that delayed recruitment of EEA1 was due to altered kinetics of PI-3-P accumulation at the phagosomal membrane. Biochemical analysis of the phosphatidylinositol phosphates on M. tuberculosis-containing phagosomes revealed that PI-3-P acquisition was markedly retarded and reduced in comparison to IgG bead-containing phagosomes. Given the role these lipids play in the regulation of phagosome maturation these findings have implications with respect to the mechanisms behind the arrest of phagosome maturation.

Original languageEnglish (US)
Pages (from-to)1627-1634
Number of pages8
JournalCellular Microbiology
Volume7
Issue number11
DOIs
StatePublished - Nov 2005
Externally publishedYes

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Phagosomes
Mycobacterium tuberculosis
Bacteria
Kinetics
Immunoglobulin G
Phosphatidylinositol Phosphates
Macrophages
Autoantigens
Phosphotransferases
Membranes
Lipids
Enzymes
Endosomes
Vacuoles
phosphatidylinositol 3-phosphate
Phagocytosis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

Kinetics of phosphatidylinositol-3-phosphate acquisition differ between lgG bead-containing phagosomes and Mycobacterium tuberculosis-containing phagosomes. / Purdy, Georgiana; Owens, Róisín M.; Bennett, Linda; Russell, David G.; Butcher, Barbara A.

In: Cellular Microbiology, Vol. 7, No. 11, 11.2005, p. 1627-1634.

Research output: Contribution to journalArticle

Purdy, Georgiana ; Owens, Róisín M. ; Bennett, Linda ; Russell, David G. ; Butcher, Barbara A. / Kinetics of phosphatidylinositol-3-phosphate acquisition differ between lgG bead-containing phagosomes and Mycobacterium tuberculosis-containing phagosomes. In: Cellular Microbiology. 2005 ; Vol. 7, No. 11. pp. 1627-1634.
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