Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani

Caleb Bashor, John M. Denu, Richard G. Brennan, Buddy Ullman

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Adenine phosphoribosyltransferase (APRT, EC 2.4.2.7) catalyzes the reversible phosphoribosylation of adenine from α-D-5-phosphoribosyl-1-pyrophosphate (PRPP) to form AMP and PPi. Three-dimensional structures of the dimeric APRT enzyme from Leishmania donovani (LdAPRT) bear many similarities to other members of the type 1 phosphoribosyltransferase family but do not reveal the structural basis for catalysis (Phillips, C. L., Ullman, B., Brennan, R. G., and Hill, C. P. (1999) EMBO J. 18, 3533-3545). To address this issue, a steady state and transient kinetic analysis of the enzyme was performed in order to determine the catalytic mechanism. Initial velocity and product inhibition studies indicated that LdAPRT follows an ordered sequential mechanism in which PRPP is the first substrate to bind and AMP is the last product to leave. This mechanistic model was substantiated by equilibrium isotope exchange and fluorescence binding studies, which provided dissociation constants for the LdAPRT - PRPP and LdAPRT - AMP binary complexes. Pre-steady-state kinetic analysis of the forward reaction revealed a burst in product formation indicating that phosphoribosyl transfer proceeds rapidly relative to some rate-limiting product release event. Transient fluorescence competition experiments enabled measurement of rates of binary complex dissociation that implicated AMP release as rate-limiting for the forward reaction. Kinetics of product ternary complex formation were evaluated using the fluorophore formycin AMP and established rate constants for pyrophosphate binding to the LdAPRT - formycin AMP complex. Taken together, these data enabled the complete formulation of an ordered bi - bi kinetic mechanism for LdAPRT in which all of the rate constants were either measured or calculated.

Original languageEnglish (US)
Pages (from-to)4020-4031
Number of pages12
JournalBiochemistry
Volume41
Issue number12
DOIs
StatePublished - Mar 26 2002

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Adenine Phosphoribosyltransferase
Leishmania donovani
Adenosine Monophosphate
Phosphoribosyl Pyrophosphate
Kinetics
Rate constants
Fluorescence
Fluorophores
Adenine
Enzymes
Catalysis
Isotopes
Ion exchange
diphosphoric acid
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bashor, C., Denu, J. M., Brennan, R. G., & Ullman, B. (2002). Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani. Biochemistry, 41(12), 4020-4031. https://doi.org/10.1021/bi0158730

Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani. / Bashor, Caleb; Denu, John M.; Brennan, Richard G.; Ullman, Buddy.

In: Biochemistry, Vol. 41, No. 12, 26.03.2002, p. 4020-4031.

Research output: Contribution to journalArticle

Bashor, C, Denu, JM, Brennan, RG & Ullman, B 2002, 'Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani', Biochemistry, vol. 41, no. 12, pp. 4020-4031. https://doi.org/10.1021/bi0158730
Bashor, Caleb ; Denu, John M. ; Brennan, Richard G. ; Ullman, Buddy. / Kinetic mechanism of adenine phosphoribosyltransferase from Leishmania donovani. In: Biochemistry. 2002 ; Vol. 41, No. 12. pp. 4020-4031.
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