Killing of cultured hepatocytes by conjugates of asialofetuin and EGF linked to the A chains of ricin or diphtheria toxin

David L. Simpson, Daniel Cawley, Harvey R. Herschman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A disulfide-linked conjugate between asialofetuin (ASF) and the toxic A chain (RTA) of ricin is as potent a toxin for cultured rat hepatocytes as our previously described conjugate between ASF and fragment A of diphtheria toxin (DTA). An RTA conjugate of epidermal growth factor (EGF) was a potent toxin for 3T3 cells. In contrast, EGF-DTA was essentially nontoxic for 3T3 cells. We have now examined the toxicity of EGF-RTA and EGF-DTA on cultured hepatocytes. The EGF-DTA conjugate, nontoxic to 3T3 cells, is also a potent toxin for hepatocytes. We also observed a decrease with time of culture in the sensitivity of hepatocytes to the ASF and EGF conjugates. This decrease is not a result of a decrease in EGF or asialoglycoprotein receptors.

Original languageEnglish (US)
Pages (from-to)469-473
Number of pages5
JournalCell
Volume29
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Diphtheria Toxin
Ricin
Epidermal Growth Factor
Hepatocytes
3T3 Cells
Rapid thermal annealing
Asialoglycoprotein Receptor
Immunotoxins
Poisons
asialofetuin
Disulfides
Toxicity
Rats

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Killing of cultured hepatocytes by conjugates of asialofetuin and EGF linked to the A chains of ricin or diphtheria toxin. / Simpson, David L.; Cawley, Daniel; Herschman, Harvey R.

In: Cell, Vol. 29, No. 2, 1982, p. 469-473.

Research output: Contribution to journalArticle

Simpson, David L. ; Cawley, Daniel ; Herschman, Harvey R. / Killing of cultured hepatocytes by conjugates of asialofetuin and EGF linked to the A chains of ricin or diphtheria toxin. In: Cell. 1982 ; Vol. 29, No. 2. pp. 469-473.
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