KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles

Virgil Muresan, Tatiana Abramson, Asya Lyass, Dirk Winter, Elena Porro, Filbert Hong, Nancy L. Chamberlin, Bruce Schnapp

Research output: Contribution to journalArticle

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Abstract

We have cloned from rat brain the cDNA encoding an 89,828-Da kinesin- related polypeptide KIF3C that is enriched in brain, retina, and lung. Immunocytochemistry of hippocampal neurons in culture shows that KIF3C is localized to cell bodies, dendrites, and, in lesser amounts, to axons. In subcellular fractionation experiments, KIF3C cofractionates with a distinct population of membrane vesicles. Native KIF3C binds to microtubules in a kinesin-like, nucleotide-dependent manner. KIF3C is most similar to mouse KIF3B and KIF3A, two closely related kinesins that are normally present as a heteromer. In sucrose density gradients, KIF3C sediments at two distinct densities, suggesting that it may be part of two different multimolecular complexes. Immunoprecipitation experiments show that KIF3C is in part associated with KIF3A, but not with KIF3B. Unlike KIF3B, a significant portion of KIF3C is not associated with KIF3A. Consistent with these biochemical properties, the distribution of KIF3C in the CNS has both similarities and differences compared with KIF3A and KIF3B. These results suggest that KIF3C is a vesicle-associated motor that functions both independently and in association with KIF3A.

Original languageEnglish (US)
Pages (from-to)637-652
Number of pages16
JournalMolecular Biology of the Cell
Volume9
Issue number3
StatePublished - 1998
Externally publishedYes

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Kinesin
Membranes
Brain
Dendrites
Immunoprecipitation
Microtubules
Axons
Sucrose
Retina
Nucleotides
Complementary DNA
Immunohistochemistry
Neurons
Lung
Peptides
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Muresan, V., Abramson, T., Lyass, A., Winter, D., Porro, E., Hong, F., ... Schnapp, B. (1998). KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles. Molecular Biology of the Cell, 9(3), 637-652.

KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles. / Muresan, Virgil; Abramson, Tatiana; Lyass, Asya; Winter, Dirk; Porro, Elena; Hong, Filbert; Chamberlin, Nancy L.; Schnapp, Bruce.

In: Molecular Biology of the Cell, Vol. 9, No. 3, 1998, p. 637-652.

Research output: Contribution to journalArticle

Muresan, V, Abramson, T, Lyass, A, Winter, D, Porro, E, Hong, F, Chamberlin, NL & Schnapp, B 1998, 'KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles', Molecular Biology of the Cell, vol. 9, no. 3, pp. 637-652.
Muresan V, Abramson T, Lyass A, Winter D, Porro E, Hong F et al. KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles. Molecular Biology of the Cell. 1998;9(3):637-652.
Muresan, Virgil ; Abramson, Tatiana ; Lyass, Asya ; Winter, Dirk ; Porro, Elena ; Hong, Filbert ; Chamberlin, Nancy L. ; Schnapp, Bruce. / KIF3C and KIF3A form a novel neuronal heteromeric kinesin that associates with membrane vesicles. In: Molecular Biology of the Cell. 1998 ; Vol. 9, No. 3. pp. 637-652.
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