Ketamine protects hippocampal neurons from anoxia in vitro

S. M. Rothman, J. H. Thurston, R. E. Hauhart, G. D. Clark, Joel Solomon

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Ketamine, a dissociative, general anesthetic, blocks the excitation produced by activating one class of excitatory amino acid receptors, the N-methyl-d-aspartate receptor in the rat. We have found that ketamine can protect hippocampal neurons in culture and slice from anoxia. When added to cultures immediately prior to anoxic exposure, ketamine prevented the neuronal destruction seen after a day of anoxia. Neurons appeared undamaged and had normal resting and action potentials. Adenosine triphosphate levels in ketamine-protected anoxic cultures were approximately two-thirds of normal controls. Ketamine also prevented the irreversible loss of the population spike seen in hippocampal slices after prolonged perfusion with anoxic buffer. These results suggest that ketamine may have therapeutic potential in preventing anoxic damage from stroke in man.

Original languageEnglish (US)
Pages (from-to)673-678
Number of pages6
JournalNeuroscience
Volume21
Issue number3
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Ketamine
Neurons
Dissociative Anesthetics
General Anesthetics
Glutamate Receptors
Membrane Potentials
Action Potentials
Hypoxia
In Vitro Techniques
Buffers
Perfusion
Adenosine Triphosphate
Stroke
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rothman, S. M., Thurston, J. H., Hauhart, R. E., Clark, G. D., & Solomon, J. (1987). Ketamine protects hippocampal neurons from anoxia in vitro. Neuroscience, 21(3), 673-678. https://doi.org/10.1016/0306-4522(87)90028-5

Ketamine protects hippocampal neurons from anoxia in vitro. / Rothman, S. M.; Thurston, J. H.; Hauhart, R. E.; Clark, G. D.; Solomon, Joel.

In: Neuroscience, Vol. 21, No. 3, 1987, p. 673-678.

Research output: Contribution to journalArticle

Rothman, SM, Thurston, JH, Hauhart, RE, Clark, GD & Solomon, J 1987, 'Ketamine protects hippocampal neurons from anoxia in vitro', Neuroscience, vol. 21, no. 3, pp. 673-678. https://doi.org/10.1016/0306-4522(87)90028-5
Rothman, S. M. ; Thurston, J. H. ; Hauhart, R. E. ; Clark, G. D. ; Solomon, Joel. / Ketamine protects hippocampal neurons from anoxia in vitro. In: Neuroscience. 1987 ; Vol. 21, No. 3. pp. 673-678.
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