JAK2/STAT3 inhibition attenuates noise-induced hearing loss

Teresa Wilson, Irina Omelchenko, Sarah Foster, Yuan Zhang, Xiao Shi, Alfred Nuttall

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Signal transducers and activators of transcription 3 (STAT3) is a stress responsive transcription factor that plays a key role in oxidative stress-mediated tissue injury. As reactive oxygen species (ROS) are a known source of damage to tissues of the inner ear following loud sound exposure, we examined the role of the Janus kinase 2 (JAK2)/STAT3 signaling pathway in noise induce hearing loss using the pathway specific inhibitor, JSI-124. Mice were exposed to a moderately damaging level of loud sound revealing the phosphorylation of STAT3 tyrosine 705 residues and nuclear localization in many cell types in the inner ear including the marginal cells of the stria vascularis, type II, III, and IV fibrocytes, spiral ganglion cells, and in the inner hair cells. Treatment of the mice with the JAK2/STAT3 inhibitor before noise exposure reduced levels of phosphorylated STAT3 Y705. We performed auditory brain stem response and distortion product otoacoustic emission measurements and found increased recovery of hearing sensitivity at two weeks after noise exposure with JAK2/STAT3 inhibition. Performance of cytocochleograms revealed improved outer hair cell survival in JSI-124 treated mice relative to control. Finally, JAK2/STAT3 inhibition reduced levels of ROS detected in outer hair cells at two hours post noise exposure. Together, these findings demonstrate that inhibiting the JAK2/STAT3 signaling pathway is protective against noise-induced cochlear tissue damage and loss of hearing sensitivity.

Original languageEnglish (US)
Pages (from-to)e108276
JournalPLoS One
Volume9
Issue number9
DOIs
StatePublished - 2014

Fingerprint

non-specific protein-tyrosine kinase
Janus Kinase 2
Noise-Induced Hearing Loss
STAT3 Transcription Factor
Audition
hearing
Acoustic noise
transcription (genetics)
Noise
Outer Auditory Hair Cells
hairs
Cells
Inner Ear
Tissue
Hearing Loss
Inner Auditory Hair Cells
cells
reactive oxygen species
ears
Reactive Oxygen Species

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

JAK2/STAT3 inhibition attenuates noise-induced hearing loss. / Wilson, Teresa; Omelchenko, Irina; Foster, Sarah; Zhang, Yuan; Shi, Xiao; Nuttall, Alfred.

In: PLoS One, Vol. 9, No. 9, 2014, p. e108276.

Research output: Contribution to journalArticle

Wilson, Teresa ; Omelchenko, Irina ; Foster, Sarah ; Zhang, Yuan ; Shi, Xiao ; Nuttall, Alfred. / JAK2/STAT3 inhibition attenuates noise-induced hearing loss. In: PLoS One. 2014 ; Vol. 9, No. 9. pp. e108276.
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