Abstract
Bradykinin (BRADY) is hypothesized to cause the “capillary leak” syndrome in patients with sepsis, trauma, and burns. Our purpose was to determine if isoproterenol (ISO) reversed a BRADY-produced accelerated loss of intravascular fluid and protein into the interstitium of skin. An increase in microvascular permeability in canine hind paw skin was sustained by a continuous femoral artery infusion of BRADY (0.2 μg/kg/min). After 2 hours of BRADY, skin lymph flow (LYM FLOW μl/min) increased nine-fold and skin lymph-to-plasma total protein concentration ratio (RTP) was substantially increased. Mean blood flow in the femoral arteries was increased four-fold by the BRADY infusion. After 2 hours of BRADY-induced increased permeability, five of the ten dogs were started on intravenous ISO(2 μg/min continuously) which increased heart rate from 182 ± 15 to 222 ±11 beats/min. ISO reversed the increase in RTP produced by the BRADY. After 8 hours of BRADY, there was less tissue albumin in the dogs given ISO (14.5 ± 2.0 vs. 29.5 ± 6.6 mg/gram dry wgt, p <0.05 unpaired t-test). ISO can reverse the sustained increase in skin microvascular permeability produced by BRADY.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1053-1064 |
| Number of pages | 12 |
| Journal | Journal of Trauma - Injury, Infection and Critical Care |
| Volume | 29 |
| Issue number | 8 |
| State | Published - 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Critical Care and Intensive Care Medicine
- Surgery
Cite this
Isoproterenol inhibits the increase in microvascular membrane permeability produced by Bradykinin. / Mullins, Richard; Malias, Mark A.; Hudgens, Russell W.
In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 29, No. 8, 1989, p. 1053-1064.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isoproterenol inhibits the increase in microvascular membrane permeability produced by Bradykinin
AU - Mullins, Richard
AU - Malias, Mark A.
AU - Hudgens, Russell W.
PY - 1989
Y1 - 1989
N2 - Bradykinin (BRADY) is hypothesized to cause the “capillary leak” syndrome in patients with sepsis, trauma, and burns. Our purpose was to determine if isoproterenol (ISO) reversed a BRADY-produced accelerated loss of intravascular fluid and protein into the interstitium of skin. An increase in microvascular permeability in canine hind paw skin was sustained by a continuous femoral artery infusion of BRADY (0.2 μg/kg/min). After 2 hours of BRADY, skin lymph flow (LYM FLOW μl/min) increased nine-fold and skin lymph-to-plasma total protein concentration ratio (RTP) was substantially increased. Mean blood flow in the femoral arteries was increased four-fold by the BRADY infusion. After 2 hours of BRADY-induced increased permeability, five of the ten dogs were started on intravenous ISO(2 μg/min continuously) which increased heart rate from 182 ± 15 to 222 ±11 beats/min. ISO reversed the increase in RTP produced by the BRADY. After 8 hours of BRADY, there was less tissue albumin in the dogs given ISO (14.5 ± 2.0 vs. 29.5 ± 6.6 mg/gram dry wgt, p <0.05 unpaired t-test). ISO can reverse the sustained increase in skin microvascular permeability produced by BRADY.
AB - Bradykinin (BRADY) is hypothesized to cause the “capillary leak” syndrome in patients with sepsis, trauma, and burns. Our purpose was to determine if isoproterenol (ISO) reversed a BRADY-produced accelerated loss of intravascular fluid and protein into the interstitium of skin. An increase in microvascular permeability in canine hind paw skin was sustained by a continuous femoral artery infusion of BRADY (0.2 μg/kg/min). After 2 hours of BRADY, skin lymph flow (LYM FLOW μl/min) increased nine-fold and skin lymph-to-plasma total protein concentration ratio (RTP) was substantially increased. Mean blood flow in the femoral arteries was increased four-fold by the BRADY infusion. After 2 hours of BRADY-induced increased permeability, five of the ten dogs were started on intravenous ISO(2 μg/min continuously) which increased heart rate from 182 ± 15 to 222 ±11 beats/min. ISO reversed the increase in RTP produced by the BRADY. After 8 hours of BRADY, there was less tissue albumin in the dogs given ISO (14.5 ± 2.0 vs. 29.5 ± 6.6 mg/gram dry wgt, p <0.05 unpaired t-test). ISO can reverse the sustained increase in skin microvascular permeability produced by BRADY.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024462393&partnerID=8YFLogxK
M3 - Article
VL - 29
SP - 1053
EP - 1064
JO - Journal of Trauma and Acute Care Surgery
JF - Journal of Trauma and Acute Care Surgery
SN - 2163-0755
IS - 8
ER -