Isolation of TSH and LH/CG receptor cDNAs from human thyroid: Regulation by tissue specific splicing

A. Lindsay Frazier, Linda S. Robbins, Philip J. Stork, Rolf Sprengel, Deborah L. Segaloff, Roger D. Cone

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid λGT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/ CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family.

Original languageEnglish (US)
Pages (from-to)1264-1276
Number of pages13
JournalMolecular Endocrinology
Volume4
Issue number8
DOIs
StatePublished - Aug 1990

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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