TY - JOUR
T1 - Isolation of TSH and LH/CG receptor cDNAs from human thyroid
T2 - Regulation by tissue specific splicing
AU - Frazier, A. Lindsay
AU - Robbins, Linda S.
AU - Stork, Philip J.
AU - Sprengel, Rolf
AU - Segaloff, Deborah L.
AU - Cone, Roger D.
PY - 1990/8
Y1 - 1990/8
N2 - A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid λGT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/ CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family.
AB - A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid λGT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/ CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family.
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U2 - 10.1210/mend-4-8-1264
DO - 10.1210/mend-4-8-1264
M3 - Article
C2 - 2293030
AN - SCOPUS:0025054351
SN - 0888-8809
VL - 4
SP - 1264
EP - 1276
JO - Molecular Endocrinology
JF - Molecular Endocrinology
IS - 8
ER -