Isolation of TSH and LH/CG receptor cDNAs from human thyroid: Regulation by tissue specific splicing

A. Lindsay Frazier, Linda S. Robbins, Philip Stork, Rolf Sprengel, Deborah L. Segaloff, Roger D. Cone

Research output: Contribution to journalArticle

137 Scopus citations

Abstract

A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid λGT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/ CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family.

Original languageEnglish (US)
Pages (from-to)1264-1276
Number of pages13
JournalMolecular Endocrinology
Volume4
Issue number8
Publication statusPublished - Aug 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Frazier, A. L., Robbins, L. S., Stork, P., Sprengel, R., Segaloff, D. L., & Cone, R. D. (1990). Isolation of TSH and LH/CG receptor cDNAs from human thyroid: Regulation by tissue specific splicing. Molecular Endocrinology, 4(8), 1264-1276.