Isolation of specific clones from nonarrayed BAC libraries through homologous recombination

Mikhail Nefedov, Lucia Carbone, Matthew Field, Jacquie Schein, Pieter J. De Jong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have developed a new approach to screen bacterial artificial chromosome (BAC) libraries by recombination selection. To test this method, we constructed an orangutan BAC library using an E. coli strain (DY380) with temperature inducible homologous recombination (HR) capability. We amplified one library segment, induced HR at 42°C to make it recombination proficient, and prepared electrocompetent cells for transformation with a kanamycin cassette to target sequences in the orangutan genome through terminal recombineering homologies. Kanamycin-resistant colonies were tested for the presence of BACs containing the targeted genes by the use of a PCR-assay to confirm the presence of the kanamycin insertion. The results indicate that this is an effective approach for screening clones. The advantage of recombination screening is that it avoids the high costs associated with the preparation, screening, and archival storage of arrayed BAC libraries. In addition, the screening can be conceivably combined with genetic engineering to create knockout and reporter constructs for functional studies.

Original languageEnglish (US)
Article number560124
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Bacterial Artificial Chromosomes
Kanamycin
Homologous Recombination
Chromosomes
Pongo
Genetic Recombination
Screening
Clone Cells
Genetic Engineering
Genes
Genetic engineering
Libraries
Genome
Escherichia coli
Costs and Cost Analysis
Assays
Polymerase Chain Reaction
Temperature
Costs

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Isolation of specific clones from nonarrayed BAC libraries through homologous recombination. / Nefedov, Mikhail; Carbone, Lucia; Field, Matthew; Schein, Jacquie; De Jong, Pieter J.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 560124, 2011.

Research output: Contribution to journalArticle

Nefedov, Mikhail ; Carbone, Lucia ; Field, Matthew ; Schein, Jacquie ; De Jong, Pieter J. / Isolation of specific clones from nonarrayed BAC libraries through homologous recombination. In: Journal of Biomedicine and Biotechnology. 2011 ; Vol. 2011.
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