Isolation of Rare Tumor Cells from Blood Cells with Buoyant Immuno-Microbubbles

Guixin Shi, Wenjin Cui, Michael Benchimol, Yu Tsueng Liu, Robert F. Mattrey, Rajesh Mukthavaram, Santosh Kesari, Sadik Esener, Dmitri Simberg

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Circulating tumor cells (CTCs) are exfoliated at various stages of cancer, and could provide invaluable information for the diagnosis and prognosis of cancers. There is an urgent need for the development of cost-efficient and scalable technologies for rare CTC enrichment from blood. Here we report a novel method for isolation of rare tumor cells from excess of blood cells using gas-filled buoyant immuno-microbubbles (MBs). MBs were prepared by emulsification of perfluorocarbon gas in phospholipids and decorated with anti-epithelial cell adhesion molecule (EpCAM) antibody. EpCAM-targeted MBs efficiently (85%) and rapidly (within 15 minutes) bound to various epithelial tumor cells suspended in cell medium. EpCAM-targeted MBs efficiently (88%) isolated frequent tumor cells that were spiked at 100,000 cells/ml into plasma-depleted blood. Anti-EpCAM MBs efficiently (>77%) isolated rare mouse breast 4T1, human prostate PC-3 and pancreatic cancer BxPC-3 cells spiked into 1, 3 and 7 ml (respectively) of plasma-depleted blood. Using EpCAM targeted MBs CTCs from metastatic cancer patients were isolated, suggesting that this technique could be developed into a valuable clinical tool for isolation, enumeration and analysis of rare cells.

Original languageEnglish (US)
Article numbere58017
JournalPLoS One
Volume8
Issue number3
DOIs
StatePublished - Mar 13 2013
Externally publishedYes

Fingerprint

microbubbles
Microbubbles
blood cells
Cell Adhesion Molecules
Tumors
Blood Cells
cell adhesion
epithelial cells
Blood
Cells
Circulating Neoplastic Cells
Neoplasms
blood plasma
neoplasms
perfluorocarbons
Gases
gases
cells
Plasmas
Fluorocarbons

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Shi, G., Cui, W., Benchimol, M., Liu, Y. T., Mattrey, R. F., Mukthavaram, R., ... Simberg, D. (2013). Isolation of Rare Tumor Cells from Blood Cells with Buoyant Immuno-Microbubbles. PLoS One, 8(3), [e58017]. https://doi.org/10.1371/journal.pone.0058017

Isolation of Rare Tumor Cells from Blood Cells with Buoyant Immuno-Microbubbles. / Shi, Guixin; Cui, Wenjin; Benchimol, Michael; Liu, Yu Tsueng; Mattrey, Robert F.; Mukthavaram, Rajesh; Kesari, Santosh; Esener, Sadik; Simberg, Dmitri.

In: PLoS One, Vol. 8, No. 3, e58017, 13.03.2013.

Research output: Contribution to journalArticle

Shi, G, Cui, W, Benchimol, M, Liu, YT, Mattrey, RF, Mukthavaram, R, Kesari, S, Esener, S & Simberg, D 2013, 'Isolation of Rare Tumor Cells from Blood Cells with Buoyant Immuno-Microbubbles', PLoS One, vol. 8, no. 3, e58017. https://doi.org/10.1371/journal.pone.0058017
Shi G, Cui W, Benchimol M, Liu YT, Mattrey RF, Mukthavaram R et al. Isolation of Rare Tumor Cells from Blood Cells with Buoyant Immuno-Microbubbles. PLoS One. 2013 Mar 13;8(3). e58017. https://doi.org/10.1371/journal.pone.0058017
Shi, Guixin ; Cui, Wenjin ; Benchimol, Michael ; Liu, Yu Tsueng ; Mattrey, Robert F. ; Mukthavaram, Rajesh ; Kesari, Santosh ; Esener, Sadik ; Simberg, Dmitri. / Isolation of Rare Tumor Cells from Blood Cells with Buoyant Immuno-Microbubbles. In: PLoS One. 2013 ; Vol. 8, No. 3.
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