Isolation of postsynaptic densities from rat brain

C. W. Cotman, Gary Banker, L. Churchill, D. Taylor

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Most synapses in the central nervous system exhibit a prominent electron opaque specialization of the postsynaptic plasma membrane called the postsynaptic density (PSD). A procedure has been devised for the isolation of PSDs which is based on their buoyant density and their insolubility in N lauroyl sarcosinate. Treatment of synaptic membranes with this detergent solubilizes most plasma membranes and detaches PSDs from the plasma membrane so that they can be purified on a density gradient. Isolated PSDs appear structurally intact and exhibit those properties which characterize them in tissue. The isolated PSDs are of the size, shape, and electron opacity of those seen in tissue; they stain with both ethanolic phosphotungstic acid and bismuth iodide uranyl lead and the fraction contains cyclic 3,5 phosphodiesterase activity. Quantitative electron microscope analysis of the PSD fraction gives an estimated purity of better than 85%. Inasmuch as the PSD is associated primarily with dendritic excitatory synapses, PSD fraction represents the distinctive plasma membrane specialization of this specific synaptic type in isolation.

Original languageEnglish (US)
JournalJournal of Cell Biology
Volume63
Issue number2
StatePublished - 1974
Externally publishedYes

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Post-Synaptic Density
Cell Membrane
Brain
Electrons
Synapses
Phosphotungstic Acid
Type 5 Cyclic Nucleotide Phosphodiesterases
Synaptic Membranes
Detergents
Coloring Agents
Central Nervous System

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cotman, C. W., Banker, G., Churchill, L., & Taylor, D. (1974). Isolation of postsynaptic densities from rat brain. Journal of Cell Biology, 63(2).

Isolation of postsynaptic densities from rat brain. / Cotman, C. W.; Banker, Gary; Churchill, L.; Taylor, D.

In: Journal of Cell Biology, Vol. 63, No. 2, 1974.

Research output: Contribution to journalArticle

Cotman, CW, Banker, G, Churchill, L & Taylor, D 1974, 'Isolation of postsynaptic densities from rat brain', Journal of Cell Biology, vol. 63, no. 2.
Cotman CW, Banker G, Churchill L, Taylor D. Isolation of postsynaptic densities from rat brain. Journal of Cell Biology. 1974;63(2).
Cotman, C. W. ; Banker, Gary ; Churchill, L. ; Taylor, D. / Isolation of postsynaptic densities from rat brain. In: Journal of Cell Biology. 1974 ; Vol. 63, No. 2.
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