Isolation of novel Src substrates

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have established and used a method to rapidly isolate tyrosine kinase substrates. The method entails inserting mammalian cDNA libraries into phage vectors. Protein production is induced, then plaque proteins are transferred to nitrocellulose and phosphorylated by the kinase of interest. Proof of principle for this technique was established by the isolation of a number of known Src substrates. We also implicated other known proteins as substrates for Src by this approach, and isolated a number of novel genes. Several of these are indeed Src substrates in mammalian cells. We have characterized further one of these novel substrates, Fish, which is a multi-domain adaptor protein.

Original languageEnglish (US)
Pages (from-to)25-28
Number of pages4
JournalBiochemical Society transactions
Volume31
Issue number1
DOIs
StatePublished - Feb 2003

Keywords

  • Phosphalidylinositol 3-phosphate
  • Phosphotyrosine
  • Phox homology (PX) domain
  • Src homology 3 (SH3) domain

ASJC Scopus subject areas

  • Biochemistry

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