Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip

Jing Cheng, Edward L. Sheldon, Lei Wu, Michael (Mike) Heller, James P. O'Connell

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

The separation and subsequent isolation of the metastatic human cervical carcinoma cell line (HeLa cells) from normal human peripheral blood cells has been achieved by exploiting their differential dielectric properties. The isolation process is carried out on a silicon chip containing a five-by-five array of microlocations. These microlocations contain underlying circular platinum electrodes with 80-μm diameters and center-to-center spacing of 200 μm. The surfaces of the electrodes and nonmetallized areas have been coated with a permeation layer to prevent the direct contact of cells with the electrode and also to minimize the nonspecific adhesion of the cells to the chip surface. An inhomogenous ac field is applied to the electrodes to create the conditions for dielectrophoretic separation of cells. Cell separation using dielectrophoresis as well as electronic lysis on a silicon chip would provide essential sample-processing steps which may be combined with a later multiplex electronic hybridization step in an integrated assay system.

Original languageEnglish (US)
Pages (from-to)2321-2326
Number of pages6
JournalAnalytical Chemistry
Volume70
Issue number11
DOIs
StatePublished - Jun 1 1998
Externally publishedYes

Fingerprint

Blood
Cells
Electrodes
Silicon
Platinum
Electrophoresis
Permeation
Dielectric properties
Assays
Adhesion
Processing

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip. / Cheng, Jing; Sheldon, Edward L.; Wu, Lei; Heller, Michael (Mike); O'Connell, James P.

In: Analytical Chemistry, Vol. 70, No. 11, 01.06.1998, p. 2321-2326.

Research output: Contribution to journalArticle

Cheng, Jing ; Sheldon, Edward L. ; Wu, Lei ; Heller, Michael (Mike) ; O'Connell, James P. / Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip. In: Analytical Chemistry. 1998 ; Vol. 70, No. 11. pp. 2321-2326.
@article{3b9b356d9e3048dda29e15f8edf57754,
title = "Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip",
abstract = "The separation and subsequent isolation of the metastatic human cervical carcinoma cell line (HeLa cells) from normal human peripheral blood cells has been achieved by exploiting their differential dielectric properties. The isolation process is carried out on a silicon chip containing a five-by-five array of microlocations. These microlocations contain underlying circular platinum electrodes with 80-μm diameters and center-to-center spacing of 200 μm. The surfaces of the electrodes and nonmetallized areas have been coated with a permeation layer to prevent the direct contact of cells with the electrode and also to minimize the nonspecific adhesion of the cells to the chip surface. An inhomogenous ac field is applied to the electrodes to create the conditions for dielectrophoretic separation of cells. Cell separation using dielectrophoresis as well as electronic lysis on a silicon chip would provide essential sample-processing steps which may be combined with a later multiplex electronic hybridization step in an integrated assay system.",
author = "Jing Cheng and Sheldon, {Edward L.} and Lei Wu and Heller, {Michael (Mike)} and O'Connell, {James P.}",
year = "1998",
month = "6",
day = "1",
doi = "10.1021/ac971274g",
language = "English (US)",
volume = "70",
pages = "2321--2326",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip

AU - Cheng, Jing

AU - Sheldon, Edward L.

AU - Wu, Lei

AU - Heller, Michael (Mike)

AU - O'Connell, James P.

PY - 1998/6/1

Y1 - 1998/6/1

N2 - The separation and subsequent isolation of the metastatic human cervical carcinoma cell line (HeLa cells) from normal human peripheral blood cells has been achieved by exploiting their differential dielectric properties. The isolation process is carried out on a silicon chip containing a five-by-five array of microlocations. These microlocations contain underlying circular platinum electrodes with 80-μm diameters and center-to-center spacing of 200 μm. The surfaces of the electrodes and nonmetallized areas have been coated with a permeation layer to prevent the direct contact of cells with the electrode and also to minimize the nonspecific adhesion of the cells to the chip surface. An inhomogenous ac field is applied to the electrodes to create the conditions for dielectrophoretic separation of cells. Cell separation using dielectrophoresis as well as electronic lysis on a silicon chip would provide essential sample-processing steps which may be combined with a later multiplex electronic hybridization step in an integrated assay system.

AB - The separation and subsequent isolation of the metastatic human cervical carcinoma cell line (HeLa cells) from normal human peripheral blood cells has been achieved by exploiting their differential dielectric properties. The isolation process is carried out on a silicon chip containing a five-by-five array of microlocations. These microlocations contain underlying circular platinum electrodes with 80-μm diameters and center-to-center spacing of 200 μm. The surfaces of the electrodes and nonmetallized areas have been coated with a permeation layer to prevent the direct contact of cells with the electrode and also to minimize the nonspecific adhesion of the cells to the chip surface. An inhomogenous ac field is applied to the electrodes to create the conditions for dielectrophoretic separation of cells. Cell separation using dielectrophoresis as well as electronic lysis on a silicon chip would provide essential sample-processing steps which may be combined with a later multiplex electronic hybridization step in an integrated assay system.

UR - http://www.scopus.com/inward/record.url?scp=0032091043&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032091043&partnerID=8YFLogxK

U2 - 10.1021/ac971274g

DO - 10.1021/ac971274g

M3 - Article

C2 - 9624903

AN - SCOPUS:0032091043

VL - 70

SP - 2321

EP - 2326

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 11

ER -