Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene

Pei-Wen Chiang, Su Qing Wang, Paul Smithivas, Woo Joo Song, Eric Crombez, Adnan Akhtar, Richard Im, Jason Greenfield, Saravanan Ramamoorthy, Margaret Van Keuren, C. Clare Blackburn, Anne Tsai, David M. Kurnit

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Abstract

To study gene regulation mediated by chromatin in mammals, we isolated the human (SUPT4H) and murine (Supt4h) counterparts of the yeast gene encoding SPT4; the product of this gene presumably interacts with the products of the mammalian homologues (which we have also cloned) of yeast SPT5 and SPT6, thereby modulating chromatin formation and activity. We isolated two different sized human SUPT4H cDNA clones (1464 and 728 nt) and one murine Supt4h (688 nt) cDNA clone; all three encode the same 117-amino- acid protein with conservation of the zinc finger motif found in SPT4. Conservation of this zinc finger motif from yeast to mouse and human implies functional importance. Although the overall sequence homology at the DNA level between the human 728-nt transcript and the murine 688-nt transcript is only 78.4%, the DNA sequence homology is 97.7% within the coding region. At the protein level, the amino acid sequences of the translated murine Supt4h and the human SUPT4H gene products are identical. The likely functional copy of SUPT4H, which has at least two introns, maps to human chromosome 17, with candidate intronless pseudogenes on chromosomes 2, 12, and 20. Buttressing the hypothesis that this is a gene required constitutively, both the human SUPT4H transcripts and the murine Supt4h transcript are expressed widely, although not at equal levels (e.g., such as most histones), in all fetal and adult tissues that we examined.

Original languageEnglish (US)
Pages (from-to)368-375
Number of pages8
JournalGenomics
Volume34
Issue number3
DOIs
StatePublished - Jun 15 1996
Externally publishedYes

Fingerprint

Yeasts
Genes
Zinc Fingers
Sequence Homology
Chromatin
Complementary DNA
Clone Cells
Chromosomes, Human, Pair 20
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 17
Pseudogenes
Chromosomes, Human, Pair 2
Human Chromosomes
Histones
Introns
Mammals
Amino Acid Sequence
Proteins
Fetus
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Chiang, P-W., Wang, S. Q., Smithivas, P., Song, W. J., Crombez, E., Akhtar, A., ... Kurnit, D. M. (1996). Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene. Genomics, 34(3), 368-375. https://doi.org/10.1006/geno.1996.0299

Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene. / Chiang, Pei-Wen; Wang, Su Qing; Smithivas, Paul; Song, Woo Joo; Crombez, Eric; Akhtar, Adnan; Im, Richard; Greenfield, Jason; Ramamoorthy, Saravanan; Van Keuren, Margaret; Blackburn, C. Clare; Tsai, Anne; Kurnit, David M.

In: Genomics, Vol. 34, No. 3, 15.06.1996, p. 368-375.

Research output: Contribution to journalArticle

Chiang, P-W, Wang, SQ, Smithivas, P, Song, WJ, Crombez, E, Akhtar, A, Im, R, Greenfield, J, Ramamoorthy, S, Van Keuren, M, Blackburn, CC, Tsai, A & Kurnit, DM 1996, 'Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene', Genomics, vol. 34, no. 3, pp. 368-375. https://doi.org/10.1006/geno.1996.0299
Chiang, Pei-Wen ; Wang, Su Qing ; Smithivas, Paul ; Song, Woo Joo ; Crombez, Eric ; Akhtar, Adnan ; Im, Richard ; Greenfield, Jason ; Ramamoorthy, Saravanan ; Van Keuren, Margaret ; Blackburn, C. Clare ; Tsai, Anne ; Kurnit, David M. / Isolation and characterization of the human and mouse homologues (SUPT4H and Supt4h) of the yeast SPT4 gene. In: Genomics. 1996 ; Vol. 34, No. 3. pp. 368-375.
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AU - Wang, Su Qing

AU - Smithivas, Paul

AU - Song, Woo Joo

AU - Crombez, Eric

AU - Akhtar, Adnan

AU - Im, Richard

AU - Greenfield, Jason

AU - Ramamoorthy, Saravanan

AU - Van Keuren, Margaret

AU - Blackburn, C. Clare

AU - Tsai, Anne

AU - Kurnit, David M.

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