Isolation and characterization of sfp: a gene that functions in the production of the lipopeptide biosurfactant, surfactin, in Bacillus subtilis

Michiko Nakano, Nathan Corbell, Jerry Besson, Peter Zuber

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

The sfp gene is required for cells of Bacillus subtilis to become producers of the lipopeptide antibiotic surfactin. sfp was isolated and its nucleotide sequence was determined. sfp was expressed in Escherichia coli and its putative product was purified for use in antibody production and in amino acid sequence analysis. The gene was plasmid-amplified in B. subtilis, where it conferred a Srf+ phenotype on sfp0 (surfactin non-producing) cells. Overproduction of Sfp in B. subtilis did not cause production of an increased amount of surfactin and resulted in the repression of a lacZ transcriptional fusion of the srfA operon, which encodes enzymes that catalyze surfactin synthesis. We propose that sfp represents an essential component of peptide synthesis systems and also plays a role, either directly or indirectly, in the regulation of surfactin biosynthesis gene expression.

Original languageEnglish (US)
Pages (from-to)313-321
Number of pages9
JournalMGG Molecular & General Genetics
Volume232
Issue number2
DOIs
StatePublished - Mar 1992
Externally publishedYes

Fingerprint

Lipopeptides
Bacillus subtilis
Genes
Protein Sequence Analysis
Operon
Antibody Formation
Plasmids
Escherichia coli
Anti-Bacterial Agents
Phenotype
Gene Expression
Peptides
Enzymes

Keywords

  • Bacillus subtilis
  • Lipopeptide antibiotic
  • sfp
  • srfA
  • Surfactin

ASJC Scopus subject areas

  • Genetics

Cite this

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AU - Corbell, Nathan

AU - Besson, Jerry

AU - Zuber, Peter

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