Isolation and Characterization of a Novel cDNA Which Identifies Both Neural‐Specific and Ubiquitously Expressed G mRNAs

Beth A. Habecker, Jennifer M. Martin, Neil M. Nathanson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Abstract: Heterotrimeric G proteins consisting of α, β, and γ subunits couple sensory, hormone, and neurotransmit‐ ter receptors to intracellular and transmembrane effectors. Several splicing variants of the Gs (the G protein that stimulates adenylyl cyclase) α subunit (G) have been described. Some of these couple receptors to stimulation of adenylyl cyclase and Ca2+ channels, whereas others encode truncated proteins whose functions are not currently defined. We describe a 1321N1 human astrocytoma cDNA clone for a novel G isoform isolated from astrocytoma cells (Gastro) that is identical to G‐1 with the exception of a novel 5’ sequence extending into the previously described exon 1 of G, a single base change, and an alternative polyadenylation site. Analysis by northern blotting and reverse transcription/PCR confirms the presence of an mRNA corresponding to this cDNA in astrocytoma cells. Additional northern analysis indicates that Gastro recognizes two novel G mRNAs in the rat: a 2.0‐kb mRNA expressed only in neural and neuroendocrine tissues and a 1.8‐kb mRNA that is ubiquitously expressed. Functional analysis of Gastro is complicated by the apparent insertion of alphoid satellite DNA into the transcription unit. The resulting cDNA encodes a truncated protein that may be translated from the methionine in exon 2 as previously described.

Original languageEnglish (US)
Pages (from-to)712-717
Number of pages6
JournalJournal of Neurochemistry
Volume61
Issue number2
DOIs
StatePublished - Aug 1993

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Keywords

  • Differential splicing
  • G protein

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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