Isolation and characterization of a low-abundance splice variant from the human cytomegalovirus major immediate-early gene region

J. A. Kerry, A. Sehgal, S. W. Barlow, V. J. Cavanaugh, K. Fish, Jay Nelson, R. M. Stenberg

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The major immediate-early (IE) gene region of human cytomegalovirus (HCMV) encodes several proteins as a result of differential RNA splicing events. By expression vector cloning of HCMV IE mRNA, we isolated and characterized a cDNA for a novel splice variant from the major IE gene region. The RNA product is a derivative of the IE55 mRNA and contains an additional splice from nucleotides 170,635 to 170,307 in the IE2 gene region (UL122), resulting in a 1.4-kb mRNA. The predicted open reading frame codes for a 164-amino- acid protein with a calculated molecular mass of 18 kDa (IE18). Mung bean nuclease analysis and PCR were used to characterize expression of IE18 mRNA in HCMV-infected cells. While the 1.4-kb mRNA was detected in infected human fibroblasts in the presence of a protein synthesis inhibitor, it was not detectable during a normal infection. However, the 1.4-kb mRNA was readily detected in infected human monocyte-derived macrophages at IE times. These results suggest that the novel IE18 mRNA exhibits cell type-specific expression indicating differential regulation of the major IE gene region in different permissive cell types.

Original languageEnglish (US)
Pages (from-to)3868-3872
Number of pages5
JournalJournal of Virology
Volume69
Issue number6
StatePublished - 1995
Externally publishedYes

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Human herpesvirus 5
Immediate-Early Genes
Cytomegalovirus
Messenger RNA
genes
RNA splicing
genetic vectors
protein synthesis inhibitors
nucleases
cells
mung beans
monocytes
fibroblasts
open reading frames
macrophages
proteins
RNA Splicing
chemical derivatives
nucleotides
Genetic Vectors

ASJC Scopus subject areas

  • Immunology

Cite this

Kerry, J. A., Sehgal, A., Barlow, S. W., Cavanaugh, V. J., Fish, K., Nelson, J., & Stenberg, R. M. (1995). Isolation and characterization of a low-abundance splice variant from the human cytomegalovirus major immediate-early gene region. Journal of Virology, 69(6), 3868-3872.

Isolation and characterization of a low-abundance splice variant from the human cytomegalovirus major immediate-early gene region. / Kerry, J. A.; Sehgal, A.; Barlow, S. W.; Cavanaugh, V. J.; Fish, K.; Nelson, Jay; Stenberg, R. M.

In: Journal of Virology, Vol. 69, No. 6, 1995, p. 3868-3872.

Research output: Contribution to journalArticle

Kerry, JA, Sehgal, A, Barlow, SW, Cavanaugh, VJ, Fish, K, Nelson, J & Stenberg, RM 1995, 'Isolation and characterization of a low-abundance splice variant from the human cytomegalovirus major immediate-early gene region', Journal of Virology, vol. 69, no. 6, pp. 3868-3872.
Kerry, J. A. ; Sehgal, A. ; Barlow, S. W. ; Cavanaugh, V. J. ; Fish, K. ; Nelson, Jay ; Stenberg, R. M. / Isolation and characterization of a low-abundance splice variant from the human cytomegalovirus major immediate-early gene region. In: Journal of Virology. 1995 ; Vol. 69, No. 6. pp. 3868-3872.
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