Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture

Grazia Pietrini, Michela Matteoli, Gary Banker, Michael J. Caplan

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The distributions of isoforms of the Na,K-ATPase a subunit were determined in mature cultured hippocampal neurons and in a polarized epithelial cell line. We find that hippocampal neurons express the α1 and α3 isoforms in the membranes of both axons and dendrites. In contrast the al and α3 proteins are exclusively basolateral when expressed endogenously or by stable transfection in renal epithelial cells. These data suggest that epithelial cells and hippocampal neurons localize these proteins by different mechanisms. These observations contrast with those made for the vesicular stomatitis virus and the influenza glycoproteins, which are polarized in both epithelial and neuronal cells.

Original languageEnglish (US)
Pages (from-to)8415-8418
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number18
StatePublished - 1992
Externally publishedYes

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Dendrites
Axons
Protein Isoforms
Epithelial Cells
Neurons
Vesicular Stomatitis
Orthomyxoviridae
Transfection
Glycoproteins
Proteins
Kidney
Cell Line
Membranes
sodium-translocating ATPase

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture. / Pietrini, Grazia; Matteoli, Michela; Banker, Gary; Caplan, Michael J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 18, 1992, p. 8415-8418.

Research output: Contribution to journalArticle

Pietrini, Grazia ; Matteoli, Michela ; Banker, Gary ; Caplan, Michael J. / Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 18. pp. 8415-8418.
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