Isoform specificity in the relationship of actin to dendritic spines

Stefanie Kaech-Petrie, Maria Fischer, Thierry Doll, Andrew Matus

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Dendritic spines contain high concentrations of actin, but neither the isoforms involved nor the mechanism of accumulation is known. In situ hybridization with specific probes established that β- and γ-cytoplasmic actins are selectively expressed at high levels by spine-bearing neurons. Transfecting cultured hippocampal neurons with epitope-tagged actin isoforms showed that cytoplasmic β- and γ-cytoplasmic actins are correctly targeted to spines; whereas α-cardiac muscle actin, which is normally absent from neurons, formed aggregates in dendrites. The transfected actin cDNAs contained only coding domains, suggesting that spine targeting involves amino acid sequences in the proteins, an interpretation supported by experiments with chimeric cDNAs in which C-terminal actin sequences were found to be determinative in spine targeting. By contrast to actin, microtubule components, including tubulin and MAP2, were restricted to the dendritic shaft domain. The close association of cytoplasmic actins with spines together with their general involvement in cell surface motility further supports the idea that actin motility-based changes in spine shape may contribute to synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)9565-9572
Number of pages8
JournalJournal of Neuroscience
Volume17
Issue number24
StatePublished - 1997
Externally publishedYes

Fingerprint

Dendritic Spines
Actins
Protein Isoforms
Spine
Neurons
Complementary DNA
Neuronal Plasticity
Tubulin
Dendrites
Microtubules
Cell Movement
In Situ Hybridization
Epitopes
Amino Acid Sequence
Myocardium

Keywords

  • Brain
  • Central nervous system
  • Cytoskeleton
  • Dendrites
  • Gene expression
  • MAP2
  • Microtubule-associated proteins
  • Microtubules
  • Neuroanatomy
  • Plasticity
  • Synapses

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Isoform specificity in the relationship of actin to dendritic spines. / Kaech-Petrie, Stefanie; Fischer, Maria; Doll, Thierry; Matus, Andrew.

In: Journal of Neuroscience, Vol. 17, No. 24, 1997, p. 9565-9572.

Research output: Contribution to journalArticle

Kaech-Petrie, S, Fischer, M, Doll, T & Matus, A 1997, 'Isoform specificity in the relationship of actin to dendritic spines', Journal of Neuroscience, vol. 17, no. 24, pp. 9565-9572.
Kaech-Petrie, Stefanie ; Fischer, Maria ; Doll, Thierry ; Matus, Andrew. / Isoform specificity in the relationship of actin to dendritic spines. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 24. pp. 9565-9572.
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