Isoflurane exposure leads to apoptosis of neurons and oligodendrocytes in 20- and 40-day old rhesus macaques

Katie J. Schenning, Kevin K. Noguchi, Lauren Drew Martin, Francesca M. Manzella, Omar H. Cabrera, Gregory Dissen, Ansgar Brambrink

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Previously we reported that a 5-hour exposure of 6-day-old (P6) rhesus macaques to isoflurane triggers robust neuron and oligodendrocyte apoptosis. In an attempt to further describe the window of vulnerability to anesthetic neurotoxicity, we exposed P20 and P40 rhesus macaques to 5. h of isoflurane anesthesia or no exposure (control animals). Brains were collected 3. h later and examined immunohistochemically to analyze neuronal and glial apoptosis. Brains exposed to isoflurane displayed neuron and oligodendrocyte apoptosis distributed throughout cortex and white matter, respectively. When combining the two age groups (P20 + P40), the animals exposed to isoflurane had 3.6 times as many apoptotic cells as the control animals. In the isoflurane group, approximately 66% of the apoptotic cells were oligodendrocytes and 34% were neurons. In comparison, in our previous studies on . P6 rhesus macaques, approximately 52% of the dying cells were glia and 48% were neurons. In conclusion, the present data suggest that the window of vulnerability for neurons is beginning to close in the P20 and P40 rhesus macaques, but continuing for oligodendrocytes.

Original languageEnglish (US)
JournalNeurotoxicology and Teratology
DOIs
StateAccepted/In press - Sep 3 2016

Fingerprint

Isoflurane
Oligodendroglia
Macaca mulatta
Neurons
Apoptosis
Animals
Neuroglia
Brain
Exposure controls
Cells
Anesthetics
Anesthesia
Age Groups
adjuvant P40

Keywords

  • Apoptosis
  • Developmental anesthesia neurotoxicity
  • Isoflurane
  • Nonhuman primates
  • Pediatric anesthesia

ASJC Scopus subject areas

  • Toxicology
  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Isoflurane exposure leads to apoptosis of neurons and oligodendrocytes in 20- and 40-day old rhesus macaques. / Schenning, Katie J.; Noguchi, Kevin K.; Martin, Lauren Drew; Manzella, Francesca M.; Cabrera, Omar H.; Dissen, Gregory; Brambrink, Ansgar.

In: Neurotoxicology and Teratology, 03.09.2016.

Research output: Contribution to journalArticle

Schenning, Katie J. ; Noguchi, Kevin K. ; Martin, Lauren Drew ; Manzella, Francesca M. ; Cabrera, Omar H. ; Dissen, Gregory ; Brambrink, Ansgar. / Isoflurane exposure leads to apoptosis of neurons and oligodendrocytes in 20- and 40-day old rhesus macaques. In: Neurotoxicology and Teratology. 2016.
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