Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content?

M. Kerker, M. A. van Dilla, A. Brunsting, J. P. Kratohvil, P. Hsu, D. S. Wang, Joe Gray, R. G. Langlois

Research output: Chapter in Book/Report/Conference proceedingChapter

35 Citations (Scopus)

Abstract

Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 μm) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10% accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.

Original languageEnglish (US)
Title of host publicationCytometry
Pages71-78
Number of pages8
Volume3
Edition2
StatePublished - 1982
Externally publishedYes

Fingerprint

Particle Size
Flow Cytometry
Coloring Agents
Fluorescence
Polystyrenes
Microspheres
Fluorescent Dyes
Polymers

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Kerker, M., van Dilla, M. A., Brunsting, A., Kratohvil, J. P., Hsu, P., Wang, D. S., ... Langlois, R. G. (1982). Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content? In Cytometry (2 ed., Vol. 3, pp. 71-78)

Is the central dogma of flow cytometry true : That fluorescence intensity is proportional to cellular dye content? / Kerker, M.; van Dilla, M. A.; Brunsting, A.; Kratohvil, J. P.; Hsu, P.; Wang, D. S.; Gray, Joe; Langlois, R. G.

Cytometry. Vol. 3 2. ed. 1982. p. 71-78.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kerker, M, van Dilla, MA, Brunsting, A, Kratohvil, JP, Hsu, P, Wang, DS, Gray, J & Langlois, RG 1982, Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content? in Cytometry. 2 edn, vol. 3, pp. 71-78.
Kerker M, van Dilla MA, Brunsting A, Kratohvil JP, Hsu P, Wang DS et al. Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content? In Cytometry. 2 ed. Vol. 3. 1982. p. 71-78
Kerker, M. ; van Dilla, M. A. ; Brunsting, A. ; Kratohvil, J. P. ; Hsu, P. ; Wang, D. S. ; Gray, Joe ; Langlois, R. G. / Is the central dogma of flow cytometry true : That fluorescence intensity is proportional to cellular dye content?. Cytometry. Vol. 3 2. ed. 1982. pp. 71-78
@inbook{4cbed801605947f79babe2a8a6187505,
title = "Is the central dogma of flow cytometry true: That fluorescence intensity is proportional to cellular dye content?",
abstract = "Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 μm) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10{\%} accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.",
author = "M. Kerker and {van Dilla}, {M. A.} and A. Brunsting and Kratohvil, {J. P.} and P. Hsu and Wang, {D. S.} and Joe Gray and Langlois, {R. G.}",
year = "1982",
language = "English (US)",
volume = "3",
pages = "71--78",
booktitle = "Cytometry",
edition = "2",

}

TY - CHAP

T1 - Is the central dogma of flow cytometry true

T2 - That fluorescence intensity is proportional to cellular dye content?

AU - Kerker, M.

AU - van Dilla, M. A.

AU - Brunsting, A.

AU - Kratohvil, J. P.

AU - Hsu, P.

AU - Wang, D. S.

AU - Gray, Joe

AU - Langlois, R. G.

PY - 1982

Y1 - 1982

N2 - Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 μm) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10% accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.

AB - Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 μm) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for the excitation and detection conditions used in flow cytometry, the relative fluorescent intensities measured for the four particle sizes are proportional to the dye content to +10% accuracy, independent of particle size. Accordingly, the central dogma of flow cytometry 'that fluorescence is proportional to cellular dye content' is valid to this accuracy for these solid, highly refractive polymer particles. Most mammalian cells are much less refractive, therefore, should conform more closely to the central dogma.

UR - http://www.scopus.com/inward/record.url?scp=0020447633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020447633&partnerID=8YFLogxK

M3 - Chapter

C2 - 7140481

AN - SCOPUS:0020447633

VL - 3

SP - 71

EP - 78

BT - Cytometry

ER -