Iron transport in Francisella in the absence of a recognizable TonB protein still requires energy generated by the proton motive force

Lidia M. Crosa, Jorge H. Crosa, Fred Heffron

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The mechanism of iron transport in Francisella is still a puzzle since none of the sequenced Francisella strains appears to encode a TonB protein, the energy transducer of the proton motive force necessary to act on the bacterial outer membrane siderophore receptor to allow the internalization of iron. In this work we demonstrate using kinetic experiments of radioactive Fe 3+ utilization, that iron uptake in Francisella novicida, although with no recognizable TonB protein, is indeed dependent on energy generated by the proton motive force. Moreover, mutants of a predicted outer membrane receptor still transport iron and are sensitive to the iron dependent antimicrobial compound streptonigrin. Our studies suggest that alternative pathways to internalize iron might exist in Francisella.

    Original languageEnglish (US)
    Pages (from-to)337-344
    Number of pages8
    JournalBioMetals
    Volume22
    Issue number2
    DOIs
    StatePublished - Apr 2009

    Fingerprint

    Francisella
    Proton-Motive Force
    Protons
    Iron
    iron
    Proteins
    energy
    proteins
    Francisella tularensis subsp. novicida
    Streptonigrin
    Membranes
    receptors
    siderophores
    Transducers
    proton-motive force
    anti-infective agents
    uptake mechanisms
    kinetics
    mutants
    Kinetics

    Keywords

    • Francisella
    • Iron transport
    • Proton motive force

    ASJC Scopus subject areas

    • Biomaterials
    • Metals and Alloys
    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Iron transport in Francisella in the absence of a recognizable TonB protein still requires energy generated by the proton motive force. / Crosa, Lidia M.; Crosa, Jorge H.; Heffron, Fred.

    In: BioMetals, Vol. 22, No. 2, 04.2009, p. 337-344.

    Research output: Contribution to journalArticle

    Crosa, Lidia M. ; Crosa, Jorge H. ; Heffron, Fred. / Iron transport in Francisella in the absence of a recognizable TonB protein still requires energy generated by the proton motive force. In: BioMetals. 2009 ; Vol. 22, No. 2. pp. 337-344.
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