IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica

Andreas J. Bäumler, Tracy L. Norris, Todd Lasco, Wolfgang Voigt, Rolf Reissbrodt, Wolfgang Rabsch, Fred Heffron

    Research output: Contribution to journalArticle

    98 Citations (Scopus)

    Abstract

    Speciation in enterobacteria involved horizontal gene transfer. Therefore, analysis of genes acquired by horizontal transfer that are present in one species but not its close relatives is expected to give insights into how new bacterial species were formed. In this study we characterize iroN, a gene located downstream of the iroBC operon in the iroA locus of Salmonella enterica serotype Typhi. Like iroBC, the iron gene is present in all phylogenetic lineages of S. enterica but is absent from closely related species such as Salmonella bongori or Escherichia coli. Comparison of the deduced amino acid sequence of iron with other proteins suggested that this gene encodes an outer membrane siderophore receptor protein. Mutational analysis in S. enterica and expression in E. coli identified a 78-kDa outer membrane protein as the iron gene product. When introduced into an E. coli fepA cir fiu aroB mutant on a cosmid, iroN mediated utilization of structurally related catecholate siderophores, including N-(2,3- dihydroxybenzoyl)-L-serine, myxochelin A, benzaldehyde-2,3- dihydroxybenzhydrazone, 2-N,6-N-bis(2,3-dihydroxybenzoyl)-L-lysine, 2-N,6-N- bis(2,3-dihydroxybenzoyl)-L-lysine amide, and enterochelin. These results suggest that the iroA locus functions in iron acquisition in S. enterica.

    Original languageEnglish (US)
    Pages (from-to)1446-1453
    Number of pages8
    JournalJournal of Bacteriology
    Volume180
    Issue number6
    StatePublished - Mar 1998

    Fingerprint

    Salmonella enterica
    Iron
    Horizontal Gene Transfer
    Escherichia coli
    Lysine
    Enterobactin
    Genes
    Siderophores
    Cosmids
    Enterobacteriaceae
    Operon
    Amides
    Salmonella
    siderophore receptors
    Amino Acid Sequence
    Membrane Proteins
    Proteins

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology
    • Immunology

    Cite this

    Bäumler, A. J., Norris, T. L., Lasco, T., Voigt, W., Reissbrodt, R., Rabsch, W., & Heffron, F. (1998). IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. Journal of Bacteriology, 180(6), 1446-1453.

    IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. / Bäumler, Andreas J.; Norris, Tracy L.; Lasco, Todd; Voigt, Wolfgang; Reissbrodt, Rolf; Rabsch, Wolfgang; Heffron, Fred.

    In: Journal of Bacteriology, Vol. 180, No. 6, 03.1998, p. 1446-1453.

    Research output: Contribution to journalArticle

    Bäumler, AJ, Norris, TL, Lasco, T, Voigt, W, Reissbrodt, R, Rabsch, W & Heffron, F 1998, 'IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica', Journal of Bacteriology, vol. 180, no. 6, pp. 1446-1453.
    Bäumler AJ, Norris TL, Lasco T, Voigt W, Reissbrodt R, Rabsch W et al. IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. Journal of Bacteriology. 1998 Mar;180(6):1446-1453.
    Bäumler, Andreas J. ; Norris, Tracy L. ; Lasco, Todd ; Voigt, Wolfgang ; Reissbrodt, Rolf ; Rabsch, Wolfgang ; Heffron, Fred. / IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. In: Journal of Bacteriology. 1998 ; Vol. 180, No. 6. pp. 1446-1453.
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