TY - JOUR
T1 - IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica
AU - Bäumler, Andreas J.
AU - Norris, Tracy L.
AU - Lasco, Todd
AU - Voigt, Wolfgang
AU - Reissbrodt, Rolf
AU - Rabsch, Wolfgang
AU - Heffron, Fred
PY - 1998/3
Y1 - 1998/3
N2 - Speciation in enterobacteria involved horizontal gene transfer. Therefore, analysis of genes acquired by horizontal transfer that are present in one species but not its close relatives is expected to give insights into how new bacterial species were formed. In this study we characterize iroN, a gene located downstream of the iroBC operon in the iroA locus of Salmonella enterica serotype Typhi. Like iroBC, the iron gene is present in all phylogenetic lineages of S. enterica but is absent from closely related species such as Salmonella bongori or Escherichia coli. Comparison of the deduced amino acid sequence of iron with other proteins suggested that this gene encodes an outer membrane siderophore receptor protein. Mutational analysis in S. enterica and expression in E. coli identified a 78-kDa outer membrane protein as the iron gene product. When introduced into an E. coli fepA cir fiu aroB mutant on a cosmid, iroN mediated utilization of structurally related catecholate siderophores, including N-(2,3- dihydroxybenzoyl)-L-serine, myxochelin A, benzaldehyde-2,3- dihydroxybenzhydrazone, 2-N,6-N-bis(2,3-dihydroxybenzoyl)-L-lysine, 2-N,6-N- bis(2,3-dihydroxybenzoyl)-L-lysine amide, and enterochelin. These results suggest that the iroA locus functions in iron acquisition in S. enterica.
AB - Speciation in enterobacteria involved horizontal gene transfer. Therefore, analysis of genes acquired by horizontal transfer that are present in one species but not its close relatives is expected to give insights into how new bacterial species were formed. In this study we characterize iroN, a gene located downstream of the iroBC operon in the iroA locus of Salmonella enterica serotype Typhi. Like iroBC, the iron gene is present in all phylogenetic lineages of S. enterica but is absent from closely related species such as Salmonella bongori or Escherichia coli. Comparison of the deduced amino acid sequence of iron with other proteins suggested that this gene encodes an outer membrane siderophore receptor protein. Mutational analysis in S. enterica and expression in E. coli identified a 78-kDa outer membrane protein as the iron gene product. When introduced into an E. coli fepA cir fiu aroB mutant on a cosmid, iroN mediated utilization of structurally related catecholate siderophores, including N-(2,3- dihydroxybenzoyl)-L-serine, myxochelin A, benzaldehyde-2,3- dihydroxybenzhydrazone, 2-N,6-N-bis(2,3-dihydroxybenzoyl)-L-lysine, 2-N,6-N- bis(2,3-dihydroxybenzoyl)-L-lysine amide, and enterochelin. These results suggest that the iroA locus functions in iron acquisition in S. enterica.
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U2 - 10.1128/jb.180.6.1446-1453.1998
DO - 10.1128/jb.180.6.1446-1453.1998
M3 - Article
C2 - 9515912
AN - SCOPUS:0031894919
SN - 0021-9193
VL - 180
SP - 1446
EP - 1453
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 6
ER -