Inward Rectification (Ih) in Immunocytochemically‐ldentified Vasopressin and Oxytocin Neurons of Guinea‐Pig Supraoptic Nucleus

Kenneth R. Erickson, Oline K. Ronnekleiv, Martin J. Kelly

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Intracellular recordings of magnocellular neurons from the supraoptic nucleus of guinea‐pigs were made with KCI/K citrate‐ and biocytin‐filled electrodes. Fifty of 99 cells exhibited a time‐dependent inward rectification (TDR). The TDR was activated during hyperpolarizing current pulses to membrane potentials more hyperpolarized than −75 mV. In voltage‐clamp recordings, an inward current appeared at voltage steps more hyperpolarized than −75 mV, with properties similar to the slow inward rectifier (Ih) described in other tissues. The Ih was blocked by 2 mM CsCI. BaCI2 (100 to 500 μM) did not block the Ih. Immunocytochemical identification of the recorded cells revealed that both vasopressin (AVP)‐ and oxytocin (OT)‐ containing neurons exhibited an Ih.

Original languageEnglish (US)
Pages (from-to)261-265
Number of pages5
JournalJournal of Neuroendocrinology
Volume2
Issue number3
DOIs
StatePublished - Jun 1990

Keywords

  • immunocytochemistry
  • inward rectification
  • oxytocin
  • supraoptic nucleus
  • vasopressin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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