Involvement of calpain in diamide-induced cataract in cultured lenses

Mitsuyoshi Azuma, Thomas R. Shearer

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32 Scopus citations

Abstract

Lenses cultured in diamide first developed outer cortical opacities followed by nuclear cataract. Lens hydration and total calcium were markedly increased by diamide. Proteolysis or crystallins were observed in nuclear cataract lenses. Calpain in the soluble fraction of lenses cultured with diamide was decreased, while calpain in the insoluble fraction was increased. Co-culture with E64d, an inhibitor of cysteine protease such as calpain, especially prevented nuclear opacities and proteolysis of crystallins, indicating that calpain was involved in cataract formation by diamide.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalFEBS Letters
Volume307
Issue number3
DOIs
StatePublished - Aug 3 1992

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Keywords

  • Calcium
  • Calpain
  • Cataract
  • Diamide
  • E64d
  • Lens culture

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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