Invited commentary: Cryopreservation as a tool for the study of selectively bred lines in rodent behavioral genetics

John Jr Crabbe, Ulrich Schneider, Jerry W. Hall, Peter Mazur

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cryopreserved embryos should survive centuries. Upon rederivation, in vitro survival is high. Upon reimplantation, in vivo survival is substantial. Genetic stability of the embryos at -196°C is theoretically absolute for several decades and likely high for centuries. Cryopreservation and rederivation services are available at a reasonable cost from commercial vendors. Preservation of a sufficient number of embryos can serve as an insurance policy against loss of the lines from several causes. In addition to the protective utility of a single banked sample, repeated preservation during the course of selection offers several advantages. Correlated responses to selection which appear late in selection may be verified by use of a cross-sectional experimental design to take a "snapshot" of the gradually developing response. After attainment of the selection limit, a generation may be chosen retrospectively which represents the best compromise between maximizing response and minimizing inbreeding irrelevant to the selected trait. Estimates of changes in gene frequencies could be derived from molecular biological analyses. An example of such an experiment in progress employing embryos from the COLD and HOT selected mouse lines is discussed.

Original languageEnglish (US)
Pages (from-to)307-312
Number of pages6
JournalBehavior Genetics
Volume23
Issue number4
DOIs
StatePublished - Jul 1993

Fingerprint

Behavioral Genetics
cryopreservation
Cryopreservation
inbred lines
rodent
embryo
Rodentia
embryo (animal)
rodents
Embryonic Structures
genetic stability
Inbreeding
Replantation
correlated responses
insurance
inbreeding
Insurance
Gene Frequency
experimental design
gene frequency

Keywords

  • correlated responses to selection
  • Cryopreservation
  • selective breeding

ASJC Scopus subject areas

  • Psychology(all)
  • Behavioral Neuroscience
  • Genetics(clinical)
  • Genetics

Cite this

Invited commentary : Cryopreservation as a tool for the study of selectively bred lines in rodent behavioral genetics. / Crabbe, John Jr; Schneider, Ulrich; Hall, Jerry W.; Mazur, Peter.

In: Behavior Genetics, Vol. 23, No. 4, 07.1993, p. 307-312.

Research output: Contribution to journalArticle

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