@inproceedings{76abbc53b5f1454fa336441445cd5945,
title = "Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy",
abstract = "Effect of hydration on the dermal collagen structure in human skin was investigated using second harmonic generation microscopy. Dog ears from the Mohs micrographic surgery department were procured for the study. Skin samples with subject aged between 58-90 years old were used in the study. Three dimensional Multiphoton (Two-photon and backward SHG) control data was acquired from the skin samples. After the control measurement, the skin tissue was either soaked in deionized water for 2 hours (Hydration) or kept at room temperature for 2 hours (Desiccation), and SHG data was acquired. The data was normalized for changes in laser power and detector gain. The collagen signal per unit volume from the dermis was calculated. The desiccated skin tissue gave higher backward SHG compared to respective control tissue, while hydration sample gave a lower backward SHG. The collagen signal decreased with increase in hydration of the dermal collagen. Hydration affected the packing of the collagen fibrils causing a change in the backward SHG signal. In this study, the use of multiphoton microscopy to study the effect of hydration on dermal structure was demonstrated in ex vivo tissue.",
author = "Ravikant Samatham and Wang, {Nicholas K.} and Jacques, {Steven L.}",
note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; Photonic Therapeutics and Diagnostics XII ; Conference date: 13-02-2016 Through 14-02-2016",
year = "2016",
doi = "10.1117/12.2214040",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Andreas Mandelis and Bernard Choi and Wong, {Brian J. F.} and Ilgner, {Justus F.} and Laura Marcu and Skala, {Melissa C.} and Nikiforos Kollias and Haishan Zeng and Kang, {Hyun Wook} and Tearney, {Guillermo J.} and Gregory, {Kenton W.} and Campagnola, {Paul J.}",
booktitle = "Photonic Therapeutics and Diagnostics XII",
}