Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy

Ravikant Samatham, Nicholas K. Wang, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Effect of hydration on the dermal collagen structure in human skin was investigated using second harmonic generation microscopy. Dog ears from the Mohs micrographic surgery department were procured for the study. Skin samples with subject aged between 58-90 years old were used in the study. Three dimensional Multiphoton (Two-photon and backward SHG) control data was acquired from the skin samples. After the control measurement, the skin tissue was either soaked in deionized water for 2 hours (Hydration) or kept at room temperature for 2 hours (Desiccation), and SHG data was acquired. The data was normalized for changes in laser power and detector gain. The collagen signal per unit volume from the dermis was calculated. The desiccated skin tissue gave higher backward SHG compared to respective control tissue, while hydration sample gave a lower backward SHG. The collagen signal decreased with increase in hydration of the dermal collagen. Hydration affected the packing of the collagen fibrils causing a change in the backward SHG signal. In this study, the use of multiphoton microscopy to study the effect of hydration on dermal structure was demonstrated in ex vivo tissue.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics XII
PublisherSPIE
Volume9689
ISBN (Electronic)9781628419245
DOIs
StatePublished - 2016
EventPhotonic Therapeutics and Diagnostics XII - San Francisco, United States
Duration: Feb 13 2016Feb 14 2016

Other

OtherPhotonic Therapeutics and Diagnostics XII
CountryUnited States
CitySan Francisco
Period2/13/162/14/16

Fingerprint

collagens
Harmonic generation
Collagen
Hydration
hydration
Microscopy
harmonic generations
Skin
Microscopic examination
Tissue
microscopy
power gain
dogs
Deionized water
ear
Mohs Surgery
surgery
Desiccation
Surgery
drying

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Samatham, R., Wang, N. K., & Jacques, S. (2016). Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy. In Photonic Therapeutics and Diagnostics XII (Vol. 9689). [96890W] SPIE. https://doi.org/10.1117/12.2214040

Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy. / Samatham, Ravikant; Wang, Nicholas K.; Jacques, Steven.

Photonic Therapeutics and Diagnostics XII. Vol. 9689 SPIE, 2016. 96890W.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Samatham, R, Wang, NK & Jacques, S 2016, Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy. in Photonic Therapeutics and Diagnostics XII. vol. 9689, 96890W, SPIE, Photonic Therapeutics and Diagnostics XII, San Francisco, United States, 2/13/16. https://doi.org/10.1117/12.2214040
Samatham, Ravikant ; Wang, Nicholas K. ; Jacques, Steven. / Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy. Photonic Therapeutics and Diagnostics XII. Vol. 9689 SPIE, 2016.
@inproceedings{76abbc53b5f1454fa336441445cd5945,
title = "Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy",
abstract = "Effect of hydration on the dermal collagen structure in human skin was investigated using second harmonic generation microscopy. Dog ears from the Mohs micrographic surgery department were procured for the study. Skin samples with subject aged between 58-90 years old were used in the study. Three dimensional Multiphoton (Two-photon and backward SHG) control data was acquired from the skin samples. After the control measurement, the skin tissue was either soaked in deionized water for 2 hours (Hydration) or kept at room temperature for 2 hours (Desiccation), and SHG data was acquired. The data was normalized for changes in laser power and detector gain. The collagen signal per unit volume from the dermis was calculated. The desiccated skin tissue gave higher backward SHG compared to respective control tissue, while hydration sample gave a lower backward SHG. The collagen signal decreased with increase in hydration of the dermal collagen. Hydration affected the packing of the collagen fibrils causing a change in the backward SHG signal. In this study, the use of multiphoton microscopy to study the effect of hydration on dermal structure was demonstrated in ex vivo tissue.",
author = "Ravikant Samatham and Wang, {Nicholas K.} and Steven Jacques",
year = "2016",
doi = "10.1117/12.2214040",
language = "English (US)",
volume = "9689",
booktitle = "Photonic Therapeutics and Diagnostics XII",
publisher = "SPIE",

}

TY - GEN

T1 - Investigation of the effect of hydration on dermal collagen in ex vivo human skin tissue using second harmonic generation microscopy

AU - Samatham, Ravikant

AU - Wang, Nicholas K.

AU - Jacques, Steven

PY - 2016

Y1 - 2016

N2 - Effect of hydration on the dermal collagen structure in human skin was investigated using second harmonic generation microscopy. Dog ears from the Mohs micrographic surgery department were procured for the study. Skin samples with subject aged between 58-90 years old were used in the study. Three dimensional Multiphoton (Two-photon and backward SHG) control data was acquired from the skin samples. After the control measurement, the skin tissue was either soaked in deionized water for 2 hours (Hydration) or kept at room temperature for 2 hours (Desiccation), and SHG data was acquired. The data was normalized for changes in laser power and detector gain. The collagen signal per unit volume from the dermis was calculated. The desiccated skin tissue gave higher backward SHG compared to respective control tissue, while hydration sample gave a lower backward SHG. The collagen signal decreased with increase in hydration of the dermal collagen. Hydration affected the packing of the collagen fibrils causing a change in the backward SHG signal. In this study, the use of multiphoton microscopy to study the effect of hydration on dermal structure was demonstrated in ex vivo tissue.

AB - Effect of hydration on the dermal collagen structure in human skin was investigated using second harmonic generation microscopy. Dog ears from the Mohs micrographic surgery department were procured for the study. Skin samples with subject aged between 58-90 years old were used in the study. Three dimensional Multiphoton (Two-photon and backward SHG) control data was acquired from the skin samples. After the control measurement, the skin tissue was either soaked in deionized water for 2 hours (Hydration) or kept at room temperature for 2 hours (Desiccation), and SHG data was acquired. The data was normalized for changes in laser power and detector gain. The collagen signal per unit volume from the dermis was calculated. The desiccated skin tissue gave higher backward SHG compared to respective control tissue, while hydration sample gave a lower backward SHG. The collagen signal decreased with increase in hydration of the dermal collagen. Hydration affected the packing of the collagen fibrils causing a change in the backward SHG signal. In this study, the use of multiphoton microscopy to study the effect of hydration on dermal structure was demonstrated in ex vivo tissue.

UR - http://www.scopus.com/inward/record.url?scp=84973326528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973326528&partnerID=8YFLogxK

U2 - 10.1117/12.2214040

DO - 10.1117/12.2214040

M3 - Conference contribution

AN - SCOPUS:84973326528

VL - 9689

BT - Photonic Therapeutics and Diagnostics XII

PB - SPIE

ER -