Investigation of Oscillatoria spongeliae-dominated bacterial communities in four dictyoceratid sponges

Christian P. Ridley, D. John Faulkner, Margo Haygood

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Certain species of marine sponges in the order Dictyoceratida harbor large populations of the cyanobacterial symbiont Oscillatoria spongeliae in the mesohyl (interior) of the sponge. We show that in four of these sponge species (Lamellodysidea herbacea, Lamellodysidea chlorea, Lendenfeldia chondrodes, and Phyllospongia papyracea) from Palau there is a consistent community of α-proteobacteria in addition to O. spongeliae that fall within the Rhodobacter group based on 16S rRNA gene analysis. Some of the α-proteobacteria in Lendenfeldia chondrodes and P. papyracea but not in the Lamellodysidea spp. contained site-specific insertions in the 16S rRNA gene. Reverse transcription-PCR experiments demonstrated that the largest insertion found in this study (63 bp) is present in the mature rRNA. Lendenfeldia chondrodes was the only sponge found to have another cyanobacterium in the tissue, a Synechocystis sp. We found that the Synechocystis sp. was present in both the pinacoderm (surface epithelial tissue) and mesohyl, in contrast to O. spongeliae, which was only found in the mesohyl through the use of specific fluorescence in situ hybridization experiments. Of the four sponge species, only P. papyracea was found to contain a significant number of γ- proteobacteria. These results demonstrate that O. spongeliae-dominated bacterial communities in different sponge species can vary considerably and increase our understanding of the bacterial communities found in marine invertebrates.

Original languageEnglish (US)
Pages (from-to)7366-7375
Number of pages10
JournalApplied and Environmental Microbiology
Volume71
Issue number11
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Oscillatoria
Porifera
bacterial communities
sponge
Proteobacteria
Synechocystis
ribosomal RNA
rRNA Genes
Palau
Rhodobacter
Rhodobacteraceae
gene
Cyanobacteria
Invertebrates
fluorescence in situ hybridization
symbiont
Fluorescence In Situ Hybridization
symbionts
Reverse Transcription
cyanobacterium

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Investigation of Oscillatoria spongeliae-dominated bacterial communities in four dictyoceratid sponges. / Ridley, Christian P.; Faulkner, D. John; Haygood, Margo.

In: Applied and Environmental Microbiology, Vol. 71, No. 11, 11.2005, p. 7366-7375.

Research output: Contribution to journalArticle

Ridley, Christian P. ; Faulkner, D. John ; Haygood, Margo. / Investigation of Oscillatoria spongeliae-dominated bacterial communities in four dictyoceratid sponges. In: Applied and Environmental Microbiology. 2005 ; Vol. 71, No. 11. pp. 7366-7375.
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