Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size

Cuicui Guo, Xia Chen, Huaidong Song, Michelle A. Maynard, Yi Zhou, Alexei V. Lobanov, Vadim N. Gladyshev, Jared J. Ganis, David Wiley, Rebecca H. Jugo, Nicholas Y. Lee, Luciana A. Castroneves, Leonard I. Zon, Thomas (Tom) Scanlan, Henry A. Feldman, Stephen A. Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Thyroid hormone is a master regulator of differentiation and growth, and its action is terminated by the enzymatic removal of an inner-ring iodine catalyzed by the selenoenzyme type 3 deiodinase (dio3). Our studies of the zebrafish reveal that the dio3 gene is duplicated in this species and that embryonic deiodination is an important determinant of embryo size. Although both dio3 paralogs encode enzymatically active proteins with high affinity for thyroid hormones, their anatomic patterns of expression are markedly divergent and only embryos with knockdown of dio3b, a biallelically expressed selenoenzyme expressed in the developing central nervous system, manifest severe thyroid hormone-dependent growth restriction at 72 hours post fertilization. This indicates that the embryonic deficiency of dio3, once considered only a placental enzyme, causes microsomia independently of placental physiology and raises the intriguing possibility that fetal abnormalities in human deiodination may present as intrauterine growth retardation. By mapping the gene structures and enzymatic properties of all four zebrafish deiodinases, we also identify dio3b as the first multiexon dio3 gene, containing a large intron separating its open reading frame from its selenocysteine insertion sequence (SECIS) element. Copyright

Original languageEnglish (US)
Pages (from-to)4069-4080
Number of pages12
JournalEndocrinology
Volume155
Issue number10
DOIs
StatePublished - Oct 1 2014

Fingerprint

Iodide Peroxidase
Zebrafish
Thyroid Hormones
Embryonic Structures
Selenocysteine
Genes
DNA Transposable Elements
Fetal Growth Retardation
Chromosome Mapping
Growth
Fertilization
Iodine
Introns
Open Reading Frames
Central Nervous System
Enzymes
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Medicine(all)

Cite this

Guo, C., Chen, X., Song, H., Maynard, M. A., Zhou, Y., Lobanov, A. V., ... Huang, S. A. (2014). Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size. Endocrinology, 155(10), 4069-4080. https://doi.org/10.1210/en.2013-2029

Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size. / Guo, Cuicui; Chen, Xia; Song, Huaidong; Maynard, Michelle A.; Zhou, Yi; Lobanov, Alexei V.; Gladyshev, Vadim N.; Ganis, Jared J.; Wiley, David; Jugo, Rebecca H.; Lee, Nicholas Y.; Castroneves, Luciana A.; Zon, Leonard I.; Scanlan, Thomas (Tom); Feldman, Henry A.; Huang, Stephen A.

In: Endocrinology, Vol. 155, No. 10, 01.10.2014, p. 4069-4080.

Research output: Contribution to journalArticle

Guo, C, Chen, X, Song, H, Maynard, MA, Zhou, Y, Lobanov, AV, Gladyshev, VN, Ganis, JJ, Wiley, D, Jugo, RH, Lee, NY, Castroneves, LA, Zon, LI, Scanlan, TT, Feldman, HA & Huang, SA 2014, 'Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size', Endocrinology, vol. 155, no. 10, pp. 4069-4080. https://doi.org/10.1210/en.2013-2029
Guo C, Chen X, Song H, Maynard MA, Zhou Y, Lobanov AV et al. Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size. Endocrinology. 2014 Oct 1;155(10):4069-4080. https://doi.org/10.1210/en.2013-2029
Guo, Cuicui ; Chen, Xia ; Song, Huaidong ; Maynard, Michelle A. ; Zhou, Yi ; Lobanov, Alexei V. ; Gladyshev, Vadim N. ; Ganis, Jared J. ; Wiley, David ; Jugo, Rebecca H. ; Lee, Nicholas Y. ; Castroneves, Luciana A. ; Zon, Leonard I. ; Scanlan, Thomas (Tom) ; Feldman, Henry A. ; Huang, Stephen A. / Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size. In: Endocrinology. 2014 ; Vol. 155, No. 10. pp. 4069-4080.
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