Intrinsic and synaptic properties of vertical cells of the mouse dorsal cochlear nucleus

Sidney P. Kuo, Hsin Wei Lu, Laurence O. Trussell

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Multiple classes of inhibitory interneurons shape the activity of principal neurons of the dorsal cochlear nucleus (DCN), a primary target of auditory nerve fibers in the mammalian brain stem. Feedforward inhibition mediated by glycinergic vertical cells (also termed tuberculoventral or corn cells) is thought to contribute importantly to the sound-evoked response properties of principal neurons, but the cellular and synaptic properties that determine how vertical cells function are unclear. We used transgenic mice in which glycinergic neurons express green fluorescent protein (GFP) to target vertical cells for whole cell patch-clamp recordings in acute slices of DCN. We found that vertical cells express diverse intrinsic spiking properties and could fire action potentials at high, sustained spiking rates. Using paired recordings, we directly examined synapses made by vertical cells onto fusiform cells, a primary DCN principal cell type. Vertical cell synapses produced unexpectedly small-amplitude unitary currents in fusiform cells, and additional experiments indicated that multiple vertical cells must be simultaneously active to inhibit fusiform cell spike output. Paired recordings also revealed that a major source of inhibition to vertical cells comes from other vertical cells.

Original languageEnglish (US)
Pages (from-to)1186-1198
Number of pages13
JournalJournal of neurophysiology
Volume108
Issue number4
DOIs
StatePublished - Aug 15 2012

Keywords

  • Brain stem
  • Glycine
  • Interneuron

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Fingerprint Dive into the research topics of 'Intrinsic and synaptic properties of vertical cells of the mouse dorsal cochlear nucleus'. Together they form a unique fingerprint.

  • Cite this