Intravenous cocaine self-administration in the C57BL/6J mouse

Nicholas J. Grahame, Tamara Phillips, Sue Burkhart-Kasch, Christopher Cunningham

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Freely behaving C57BL/6J mice with intrajugular catheters were trained to nose-poke for cocaine (0.75 mg/kg per 5-μ1 infusion) under a fixed-ratio-10 schedule of reinforcement. Mice were given a choice between two nose-poke holes on opposite sides of the apparatus. Nose-pokes by experimental (O) subjects (operant group) were reinforced on only one side and reinforcer delivery coincided with the onset of a 10-s time-out light stimulus. Drug delivery to control subjects (yoked group) was determined by the behavior of O mice. Nose-poke rate increased in O subjects, whereas yoked subjects did not acquire the nose-poking response. Moreover, nose-poking was selective for the cocaine-paired side in O subjects. When saline infusions were substituted for cocaine (i.e., extinction), nose-poking in O subjects decreased, whereas yoked controls were unaffected. O subjects developed a preference for the drug-associated side of the apparatus during extinction. Overall, these data offer strong evidence of cocaine-directed behavior in the C57BL/6 inbred mouse strain. More generally, these findings support the feasibility of using intravenous self-administration to assess reinforcement in genetically well-defined populations.

Original languageEnglish (US)
Pages (from-to)827-834
Number of pages8
JournalPharmacology, Biochemistry and Behavior
Volume51
Issue number4
DOIs
StatePublished - 1995

Fingerprint

Self Administration
Inbred C57BL Mouse
Cocaine
Nose
Reinforcement
Catheters
Drug delivery
Reinforcement Schedule
Inbred Strains Mice
Pharmaceutical Preparations
Intravenous Administration
Light
Population

Keywords

  • C57BL/6J mice
  • Cocaine
  • Intravenous self-administration
  • Locomotor activity
  • Place preference
  • Reinforcement
  • Yoked control

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Behavioral Neuroscience
  • Biological Psychiatry
  • Pharmacology
  • Toxicology

Cite this

Intravenous cocaine self-administration in the C57BL/6J mouse. / Grahame, Nicholas J.; Phillips, Tamara; Burkhart-Kasch, Sue; Cunningham, Christopher.

In: Pharmacology, Biochemistry and Behavior, Vol. 51, No. 4, 1995, p. 827-834.

Research output: Contribution to journalArticle

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