Intrastriatal B-cell administration limits infarct size after stroke in B-cell deficient mice

Yingxin Chen, Sheetal Bodhankar, Stephanie J. Murphy, Arthur Vandenbark, Nabil Alkayed, Halina Offner

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Recent evidence emphasizes B-cells as a major regulatory cell type that plays an important role in limiting the pathogenic effects of ischemic stroke. The aim of the current study was to extend this initial observation to specifically examine the infiltration of regulatory B-cells and to determine if the effect of B-cells to limit the inflammatory response to cerebral ischemia is mediated by their action centrally or peripherally. Our data demonstrate the increased presence of a regulatory B-cell subset in the affected hemisphere of wild-type mice after middle cerebral artery occlusion (MCAO). We further explored the use of a novel method of stereotaxic cell delivery to bypass the blood brain barrier (BBB) and introduce CD19+ B-cells directly into the striatum as compared to peripheral administration of B-cells. Infarct volumes after 60 minutes of MCAO and 48 hours of reperfusion were determined in B-cell deficient μMT-/- mice with and without replacement of either B-cells or medium. Infarct size was significantly decreased in cerebral cortex after intrastriatal transfer of 100,000 B-cells to μMT-/- mice vs. controls, with a comparable effect on infarct size as obtained by 50 million B-cells transferred intraperitoneally. These findings support the hypothesis that B-cells play a protective role against ischemic brain injury, and suggest that B-cells may serve as a novel therapeutic agent for modulating the immune response in central nervous system inflammation after stroke.

Original languageEnglish (US)
Pages (from-to)487-493
Number of pages7
JournalMetabolic Brain Disease
Volume27
Issue number4
DOIs
StatePublished - Dec 2012

Fingerprint

B-Lymphocytes
Stroke
Cells
Regulatory B-Lymphocytes
Middle Cerebral Artery Infarction
B-Lymphocyte Subsets
Brain Ischemia
Blood-Brain Barrier
Cerebral Cortex
Brain Injuries
Reperfusion
Neurology
Infiltration
Central Nervous System
Observation
Brain
Inflammation

Keywords

  • μMT mice
  • Experimental stroke
  • Intrastriatal transfer
  • Regulatory B-cells

ASJC Scopus subject areas

  • Clinical Neurology
  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Intrastriatal B-cell administration limits infarct size after stroke in B-cell deficient mice. / Chen, Yingxin; Bodhankar, Sheetal; Murphy, Stephanie J.; Vandenbark, Arthur; Alkayed, Nabil; Offner, Halina.

In: Metabolic Brain Disease, Vol. 27, No. 4, 12.2012, p. 487-493.

Research output: Contribution to journalArticle

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