Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat

Gregory Dissen, H. E. Lara, V. Leyton, A. Paredes, D. F. Hill, M. E. Costa, A. Martinez-Serrano, Sergio Ojeda

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    A single injection of estradiol valerate induces a form of cystic ovary resembling some aspects of the human polycystic ovarian syndrome. Preceding the development of follicular cysts, there is an increase in intraovarian synthesis of nerve growth factor (NGF) and the low affinity NGF receptor (p75 NGFR). Selective blockade of NGF actions and p75 NGFR synthesis in the ovary restored estrous cyclicity and ovulatory capacity in estradiol valerate-treated rats, suggesting that an increase in NGF-dependent, p75 NGFR-mediated actions within the ovary contributes to the development of cystic ovarian disease. We have tested this hypothesis by grafting NGF-producing neural progenitor cells into the ovary of juvenile rats that have been induced to ovulate precociously by a single injection of PMSG. The NGF-producing cells, detected by their content of immunoreactive p75 NGFR material, were found scattered throughout the ovary with some of them infiltrating the granulosa cell compartment of large, precystic follicles. Ovarian NGF content was 2-fold higher than in the ovary of rats receiving control cells. Estrous cyclicity was disrupted, with the animals showing prolonged periods of persistent estrus, and an almost continuous background of vaginal cornified cells at other phases of the estrous cycle. Morphometric analysis revealed that the presence of NGF-producing cells neither reduced the total number of corpora lutea per ovary nor significantly increased the formation of follicular cysts. However, the ovaries receiving these cells showed an increased incidence of precystic, type III follicles, accompanied by a reduced number of healthy antral follicles, and an increased size of both healthy and atretic follicles. These changes in follicular dynamics were accompanied by a selective increase in serum androstenedione levels. The results show that an abnormally elevated production of NGF within the ovary suffices to initiate several of the structural and functional alterations associated with the development of follicular cysts in the rat ovary.

    Original languageEnglish (US)
    Pages (from-to)1073-1082
    Number of pages10
    JournalEndocrinology
    Volume141
    Issue number3
    DOIs
    StatePublished - 2000

    Fingerprint

    Nerve Growth Factor
    Periodicity
    Androgens
    Ovary
    estradiol valerate
    Follicular Cyst
    Nerve Growth Factor Receptor
    Ovarian Diseases
    Injections
    Ovarian Follicle
    Estrous Cycle
    Androstenedione
    Granulosa Cells
    Polycystic Ovary Syndrome
    Corpus Luteum
    Estrus
    Stem Cells
    Incidence

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat. / Dissen, Gregory; Lara, H. E.; Leyton, V.; Paredes, A.; Hill, D. F.; Costa, M. E.; Martinez-Serrano, A.; Ojeda, Sergio.

    In: Endocrinology, Vol. 141, No. 3, 2000, p. 1073-1082.

    Research output: Contribution to journalArticle

    Dissen, G, Lara, HE, Leyton, V, Paredes, A, Hill, DF, Costa, ME, Martinez-Serrano, A & Ojeda, S 2000, 'Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat', Endocrinology, vol. 141, no. 3, pp. 1073-1082. https://doi.org/10.1210/en.141.3.1073
    Dissen, Gregory ; Lara, H. E. ; Leyton, V. ; Paredes, A. ; Hill, D. F. ; Costa, M. E. ; Martinez-Serrano, A. ; Ojeda, Sergio. / Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat. In: Endocrinology. 2000 ; Vol. 141, No. 3. pp. 1073-1082.
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    AU - Lara, H. E.

    AU - Leyton, V.

    AU - Paredes, A.

    AU - Hill, D. F.

    AU - Costa, M. E.

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    AU - Ojeda, Sergio

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