Intraocular Lymphoma: Immunopathologic Analysis of Vitreous Biopsy Specimens

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Immunologic analysis of cell surface markers (immunophenotyping) has become a standard procedure in the evaluation of systemic lymphomas. However, attempts to apply these techniques to intraocular lymphoma have not been uniformly successful. We successfully immunophenotyped five consecutive cases of intraocular lymphoma using immunoperoxidase surface marker analysis of cytocentrifuged specimens in two cases and flow cytometry in three. In all five cases, a monoclonal B-cell population was unequivocally present. Contrary to previous reports, we found surface marker analysis of vitreous biopsy specimens to be helpful in the diagnosis and treatment of intraocular lymphoma. Not only did it support the cytologic diagnosis but it allowed comparison of the immunophenotype of vitreous infiltrates with that of previous or subsequent lymphomatous lesions from nonocular sites.

Original languageEnglish (US)
Pages (from-to)1455-1458
Number of pages4
JournalArchives of Ophthalmology
Volume110
Issue number10
DOIs
StatePublished - 1992

Fingerprint

Intraocular Lymphoma
Biopsy
Immunophenotyping
Lymphoma
Flow Cytometry
B-Lymphocytes
Population

ASJC Scopus subject areas

  • Ophthalmology

Cite this

@article{f8f65f94fd5f4db3843561e83989c0c2,
title = "Intraocular Lymphoma: Immunopathologic Analysis of Vitreous Biopsy Specimens",
abstract = "Immunologic analysis of cell surface markers (immunophenotyping) has become a standard procedure in the evaluation of systemic lymphomas. However, attempts to apply these techniques to intraocular lymphoma have not been uniformly successful. We successfully immunophenotyped five consecutive cases of intraocular lymphoma using immunoperoxidase surface marker analysis of cytocentrifuged specimens in two cases and flow cytometry in three. In all five cases, a monoclonal B-cell population was unequivocally present. Contrary to previous reports, we found surface marker analysis of vitreous biopsy specimens to be helpful in the diagnosis and treatment of intraocular lymphoma. Not only did it support the cytologic diagnosis but it allowed comparison of the immunophenotype of vitreous infiltrates with that of previous or subsequent lymphomatous lesions from nonocular sites.",
author = "David Wilson and Rita Braziel and Rosenbaum, {James (Jim)}",
year = "1992",
doi = "10.1001/archopht.1992.01080220117032",
language = "English (US)",
volume = "110",
pages = "1455--1458",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "10",

}

TY - JOUR

T1 - Intraocular Lymphoma

T2 - Immunopathologic Analysis of Vitreous Biopsy Specimens

AU - Wilson, David

AU - Braziel, Rita

AU - Rosenbaum, James (Jim)

PY - 1992

Y1 - 1992

N2 - Immunologic analysis of cell surface markers (immunophenotyping) has become a standard procedure in the evaluation of systemic lymphomas. However, attempts to apply these techniques to intraocular lymphoma have not been uniformly successful. We successfully immunophenotyped five consecutive cases of intraocular lymphoma using immunoperoxidase surface marker analysis of cytocentrifuged specimens in two cases and flow cytometry in three. In all five cases, a monoclonal B-cell population was unequivocally present. Contrary to previous reports, we found surface marker analysis of vitreous biopsy specimens to be helpful in the diagnosis and treatment of intraocular lymphoma. Not only did it support the cytologic diagnosis but it allowed comparison of the immunophenotype of vitreous infiltrates with that of previous or subsequent lymphomatous lesions from nonocular sites.

AB - Immunologic analysis of cell surface markers (immunophenotyping) has become a standard procedure in the evaluation of systemic lymphomas. However, attempts to apply these techniques to intraocular lymphoma have not been uniformly successful. We successfully immunophenotyped five consecutive cases of intraocular lymphoma using immunoperoxidase surface marker analysis of cytocentrifuged specimens in two cases and flow cytometry in three. In all five cases, a monoclonal B-cell population was unequivocally present. Contrary to previous reports, we found surface marker analysis of vitreous biopsy specimens to be helpful in the diagnosis and treatment of intraocular lymphoma. Not only did it support the cytologic diagnosis but it allowed comparison of the immunophenotype of vitreous infiltrates with that of previous or subsequent lymphomatous lesions from nonocular sites.

UR - http://www.scopus.com/inward/record.url?scp=0026793530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026793530&partnerID=8YFLogxK

U2 - 10.1001/archopht.1992.01080220117032

DO - 10.1001/archopht.1992.01080220117032

M3 - Article

C2 - 1417546

AN - SCOPUS:0026793530

VL - 110

SP - 1455

EP - 1458

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 10

ER -