Intracellular transport dynamics of endosomes containing DNA polyplexes along the microtubule network

Rajan P. Kulkarni, Kenneth Castelino, Arun Majumdar, Scott E. Fraser

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

We have explored the transport of DNA polyplexes enclosed in endosomes within the cellular environment by multiple particle tracking (MPT). The polyplex-loaded endosomes demonstrate enhanced diffusion at short timescales (t < 7 s) with their mean-square displacement (MSD) 〈Δx(t)2〉 scaling as t1.25. For longer time intervals they exhibit subdiffusive transport and have an MSD scaling as t0.7. This crossover from an enhanced diffusion to a subdiffusive regime can be explained by considering the action of motor proteins that actively transport these endosomes along the cellular microtubule network and the thermal bending modes of the microtubule network itself.

Original languageEnglish (US)
Pages (from-to)L42-L44
JournalBiophysical Journal
Volume90
Issue number5
DOIs
StatePublished - Mar 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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