Intracellular trafficking of fluorescently tagged proteins associated with pathogenesis in Candida albicans

Samuel A. Lee, Zachary Khalique, Cheryl A. Gale, Brian Wong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Proteins that enter the secretory pathway play important roles in virulence and pathogenesis in Candida albicans, but our understanding of the trafficking of these proteins is in its early stages. In Saccharomyces cerevisiae, dominant negative alleles of YPT1 and SEC4 interrupt secretory traffic at pre- and post-Golgi steps, respectively. We therefore used a dominant negative genetic approach to examine the intracellular trafficking of several proteins associated with virulence or azole resistance. When the dominant negative ypt1(N121I) allele of C. albicans was overexpressed, yellow-fluorescent protein (YFP) tagged forms of two plasma membrane transporters (Cdr1p and Ftr1p) and the vacuolar membrane ABC transporter Mlt1p accumulated in intracellular structures that appeared related to the ER, but localization of Cdc10p and Int1p was unaffected. When the dominant negative sec4(S28N) allele of C. albicans was overexpressed, Cdr1p and Ftr1p accumulated intracellularly, and localization of Mlt1p, Cdc10p and Int1p was unaffected. These results imply that (i) Cdr1p and Ftr1p are transported to the plasma membrane by the general secretory pathway, (ii) Mlt1p enters the secretory pathway but is diverted to the vacuole at an early post-Golgi step, and (iii) like Cdc10p, Int1p does not enter the general secretory pathway.

Original languageEnglish (US)
Pages (from-to)423-430
Number of pages8
JournalMedical Mycology
Volume43
Issue number5
DOIs
StatePublished - Aug 2005
Externally publishedYes

Fingerprint

Secretory Pathway
Candida albicans
pathogenesis
protein transport
alleles
Membrane Transport Proteins
Alleles
Protein Transport
virulence
plasma membrane
Virulence
azoles
Proteins
ABC transporters
proteins
Cell Membrane
traffic
Azoles
ATP-Binding Cassette Transporters
vacuoles

Keywords

  • Candida albicans
  • Protein secretion
  • SECY
  • Yellow fluorescent protein
  • YPT1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

Intracellular trafficking of fluorescently tagged proteins associated with pathogenesis in Candida albicans. / Lee, Samuel A.; Khalique, Zachary; Gale, Cheryl A.; Wong, Brian.

In: Medical Mycology, Vol. 43, No. 5, 08.2005, p. 423-430.

Research output: Contribution to journalArticle

Lee, Samuel A. ; Khalique, Zachary ; Gale, Cheryl A. ; Wong, Brian. / Intracellular trafficking of fluorescently tagged proteins associated with pathogenesis in Candida albicans. In: Medical Mycology. 2005 ; Vol. 43, No. 5. pp. 423-430.
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