Intracellular sphingosine releases calcium from lysosomes

Doris Höglinger, Per Haberkant, Auxiliadora Aguilera-Romero, Howard Riezman, Forbes D. Porter, Frances M. Platt, Antony Galione, Carsten Schultz

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

To elucidate new functions of sphingosine (Sph), we demonstrate that the spontaneous elevation of intracellular Sph levels via caged Sph leads to a significant and transient calcium release from acidic stores that is independent of sphingosine 1-phosphate, extracellular and ER calcium levels. This photo-induced Sph-driven calcium release requires the two-pore channel 1 (TPC1) residing on endosomes and lysosomes. Further, uncaging of Sph leads to the translocation of the autophagy-relevant transcription factor EB (TFEB) to the nucleus specifically after lysosomal calcium release. We confirm that Sph accumulates in late endosomes and lysosomes of cells derived from Niemann-Pick disease type C (NPC) patients and demonstrate a greatly reduced calcium release upon Sph uncaging. We conclude that sphingosine is a positive regulator of calcium release from acidic stores and that understanding the interplay between Sph homeostasis, calcium signaling and autophagy will be crucial in developing new therapies for lipid storage disorders such as NPC.

Original languageEnglish (US)
Article numbere10616
JournaleLife
Volume4
Issue numberNOVEMBER2015
DOIs
StatePublished - Nov 27 2015
Externally publishedYes

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Sphingosine
Lysosomes
Calcium
Endosomes
Autophagy
Type C Niemann-Pick Disease
Calcium Signaling
Homeostasis
Transcription Factors
Lipids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Höglinger, D., Haberkant, P., Aguilera-Romero, A., Riezman, H., Porter, F. D., Platt, F. M., ... Schultz, C. (2015). Intracellular sphingosine releases calcium from lysosomes. eLife, 4(NOVEMBER2015), [e10616]. https://doi.org/10.7554/eLife.10616

Intracellular sphingosine releases calcium from lysosomes. / Höglinger, Doris; Haberkant, Per; Aguilera-Romero, Auxiliadora; Riezman, Howard; Porter, Forbes D.; Platt, Frances M.; Galione, Antony; Schultz, Carsten.

In: eLife, Vol. 4, No. NOVEMBER2015, e10616, 27.11.2015.

Research output: Contribution to journalArticle

Höglinger, D, Haberkant, P, Aguilera-Romero, A, Riezman, H, Porter, FD, Platt, FM, Galione, A & Schultz, C 2015, 'Intracellular sphingosine releases calcium from lysosomes', eLife, vol. 4, no. NOVEMBER2015, e10616. https://doi.org/10.7554/eLife.10616
Höglinger D, Haberkant P, Aguilera-Romero A, Riezman H, Porter FD, Platt FM et al. Intracellular sphingosine releases calcium from lysosomes. eLife. 2015 Nov 27;4(NOVEMBER2015). e10616. https://doi.org/10.7554/eLife.10616
Höglinger, Doris ; Haberkant, Per ; Aguilera-Romero, Auxiliadora ; Riezman, Howard ; Porter, Forbes D. ; Platt, Frances M. ; Galione, Antony ; Schultz, Carsten. / Intracellular sphingosine releases calcium from lysosomes. In: eLife. 2015 ; Vol. 4, No. NOVEMBER2015.
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