Intracellular paired agent imaging enables improved evaluation of tyrosine kinase inhibitor target engagement

Allison Solanki, Lei Wang, Jesse Korber, Nathan McMahon, Kenneth Tichauer, Kimberley S. Samkoe, Summer L. Gibbs

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Targeting the aberrant epidermal growth factor receptor (EGFR) signaling pathway is an attractive choice for many cancers (e.g., non-small cell lung carcinoma (NSCLC) and head and neck squamous cell carcinoma (HNSCC)). Despite the development of promising therapeutics, incomplete target engagement and acquired resistance (e.g., mutagenesis and intracellular signaling pathway rewiring) ensure that curative options still elude patients. To address limitations posed by standard drug evaluation assays (e.g., western blot, bulk plasma monitoring, immunohistochemistry), we have developed a novel dynamic, fluorescence-based platform termed intracellular paired agent imaging (iPAI). iPAI quantifies intracellular protein target engagement using two matched small-molecule, cell membrane-permeable agents: one targeted to the protein of interest and one untargeted, which accounts for non-specific therapeutic uptake. Currently, our iPAI panel includes successfully characterized tyrosine kinase inhibitors targeting the kinase binding domain of numerous proteins in the EGFR pathway, including erlotinib (EGFR). Here, we present a pharmacokinetic uptake study using our novel iPAI erlotinib reagents: a targeted erlotinib probed conjugated to silicon tetramethylrhodamine (Erl- SiTMR-T) and an untargeted reagent conjugated to tetramethylrhodaime (Erl-TMR-UT). An initial uptake study in a cell derived xenograft (CDX) model of NSCLC was performed by administering the Erl iPAI reagents systemically via tail vein injection, where drug uptake was quantified in the tumor over time. Excitingly, evidence of heterogeneous uptake was observed in the iPAI injected cohort, displaying distinct drug-uptake within a single tumor. Characterization of additional iPAI agents targeting downstream effectors (e.g., AKT, PI3K, MEK and ERK) is ongoing and will allow us to visualize complex drug-target interactions and quantify their downstream signaling partners during treatment regimens for NSCLC and other cancers. Together, we anticipate these iPAI probes will improve understanding of current limitations in personalized cancer therapy.

Original languageEnglish (US)
Title of host publicationVisualizing and Quantifying Drug Distribution in Tissue IV
EditorsKin Foong Chan, Conor L. Evans
PublisherSPIE
ISBN (Electronic)9781510632011
DOIs
StatePublished - Jan 1 2020
EventVisualizing and Quantifying Drug Distribution in Tissue IV 2020 - San Francisco, United States
Duration: Feb 1 2020 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11219
ISSN (Print)1605-7422

Conference

ConferenceVisualizing and Quantifying Drug Distribution in Tissue IV 2020
CountryUnited States
CitySan Francisco
Period2/1/20 → …

Keywords

  • Cancer biology
  • Drug-target engagement
  • Epidermal growth factor receptor
  • Fluorescence imaging
  • Intracellular paired agent imaging
  • Tyrosine kinase inhibitor

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Solanki, A., Wang, L., Korber, J., McMahon, N., Tichauer, K., Samkoe, K. S., & Gibbs, S. L. (2020). Intracellular paired agent imaging enables improved evaluation of tyrosine kinase inhibitor target engagement. In K. F. Chan, & C. L. Evans (Eds.), Visualizing and Quantifying Drug Distribution in Tissue IV [112190F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11219). SPIE. https://doi.org/10.1117/12.2546234