Intra-arterial interleukin-12 gene delivery combined with chemoembolization: Anti-tumor effect in a rabbit hepatocellular carcinoma (HCC) model

Xiangwen Xia, Xin Li, Gansheng Feng, Chuansheng Zheng, Huimin Liang, Guofeng Zhou

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Interleukin-12 (IL-12), a cytokine naturally secreted by activated dendritic cells and monocytes/macrophages, is known as a key anti-tumor agent in many tumor models, including hepatocellular carcinoma (HCC) models. Purpose: To evaluate the anti-tumor effect of intra-arterial IL-12 gene delivery alone and in combination with transcatheter arterial chemoembolization (TACE) in rabbit VX2 liver cancer model. Material and Methods: Rabbits with VX2 liver tumors were randomized into four groups, eight in each group. After laparotomy and insertion of a 30-gauge needle into the proper hepatic artery, the following interventional procedure protocols were applied: 0.9% saline solution (group A, control), TACE (group B, TACE alone, lipiodol + mitomycin), intra-arterial interleukin-12 gene infusion (group C, IL-12 alone), and intra-arterial interleukin-12 gene infusion in combination with TACE (group D, IL-12 plus TACE). Growth ratio was estimated by computed tomography. To analyze apoptotic index, tumor tissues were explanted for terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining, 14 days after therapy. Results: Significant differences of the relative tumor growth ratio were observed in TACE alone group and IL-12 plus TACE group in comparison with control (P <0.05, ANOVA, Tukey's HSD correction) but not between IL-12 alone and control, or IL-12 plus TACE group and TACE alone group (P > 0.05). Significant changes of the apoptotic index were observed in group D in comparison with remaining three groups (P <0.05). The difference between group C and group A was not significant statistically (P > 0.05). Conclusion: Intra-arterial interleukin-12 gene therapy combined with TACE has a potent anti-tumor effect in rabbit VX2 liver cancer in comparison with TACE alone.

Original languageEnglish (US)
Pages (from-to)684-689
Number of pages6
JournalActa Radiologica
Volume54
Issue number6
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Interleukin-12
Hepatocellular Carcinoma
Rabbits
Genes
Neoplasms
Liver Neoplasms
Ethiodized Oil
DNA Nucleotidylexotransferase
Hepatic Artery
Mitomycin
Growth
Sodium Chloride
Genetic Therapy
Laparotomy
Dendritic Cells
Needles
Monocytes
Macrophages
Tomography
Staining and Labeling

Keywords

  • Anti-tumor
  • Apoptotic index
  • Chemoembolization
  • Gene therapy
  • Interleukin-12
  • Liver cancer

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Medicine(all)

Cite this

Intra-arterial interleukin-12 gene delivery combined with chemoembolization : Anti-tumor effect in a rabbit hepatocellular carcinoma (HCC) model. / Xia, Xiangwen; Li, Xin; Feng, Gansheng; Zheng, Chuansheng; Liang, Huimin; Zhou, Guofeng.

In: Acta Radiologica, Vol. 54, No. 6, 2013, p. 684-689.

Research output: Contribution to journalArticle

Xia, Xiangwen ; Li, Xin ; Feng, Gansheng ; Zheng, Chuansheng ; Liang, Huimin ; Zhou, Guofeng. / Intra-arterial interleukin-12 gene delivery combined with chemoembolization : Anti-tumor effect in a rabbit hepatocellular carcinoma (HCC) model. In: Acta Radiologica. 2013 ; Vol. 54, No. 6. pp. 684-689.
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abstract = "Background: Interleukin-12 (IL-12), a cytokine naturally secreted by activated dendritic cells and monocytes/macrophages, is known as a key anti-tumor agent in many tumor models, including hepatocellular carcinoma (HCC) models. Purpose: To evaluate the anti-tumor effect of intra-arterial IL-12 gene delivery alone and in combination with transcatheter arterial chemoembolization (TACE) in rabbit VX2 liver cancer model. Material and Methods: Rabbits with VX2 liver tumors were randomized into four groups, eight in each group. After laparotomy and insertion of a 30-gauge needle into the proper hepatic artery, the following interventional procedure protocols were applied: 0.9{\%} saline solution (group A, control), TACE (group B, TACE alone, lipiodol + mitomycin), intra-arterial interleukin-12 gene infusion (group C, IL-12 alone), and intra-arterial interleukin-12 gene infusion in combination with TACE (group D, IL-12 plus TACE). Growth ratio was estimated by computed tomography. To analyze apoptotic index, tumor tissues were explanted for terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining, 14 days after therapy. Results: Significant differences of the relative tumor growth ratio were observed in TACE alone group and IL-12 plus TACE group in comparison with control (P <0.05, ANOVA, Tukey's HSD correction) but not between IL-12 alone and control, or IL-12 plus TACE group and TACE alone group (P > 0.05). Significant changes of the apoptotic index were observed in group D in comparison with remaining three groups (P <0.05). The difference between group C and group A was not significant statistically (P > 0.05). Conclusion: Intra-arterial interleukin-12 gene therapy combined with TACE has a potent anti-tumor effect in rabbit VX2 liver cancer in comparison with TACE alone.",
keywords = "Anti-tumor, Apoptotic index, Chemoembolization, Gene therapy, Interleukin-12, Liver cancer",
author = "Xiangwen Xia and Xin Li and Gansheng Feng and Chuansheng Zheng and Huimin Liang and Guofeng Zhou",
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T1 - Intra-arterial interleukin-12 gene delivery combined with chemoembolization

T2 - Anti-tumor effect in a rabbit hepatocellular carcinoma (HCC) model

AU - Xia, Xiangwen

AU - Li, Xin

AU - Feng, Gansheng

AU - Zheng, Chuansheng

AU - Liang, Huimin

AU - Zhou, Guofeng

PY - 2013

Y1 - 2013

N2 - Background: Interleukin-12 (IL-12), a cytokine naturally secreted by activated dendritic cells and monocytes/macrophages, is known as a key anti-tumor agent in many tumor models, including hepatocellular carcinoma (HCC) models. Purpose: To evaluate the anti-tumor effect of intra-arterial IL-12 gene delivery alone and in combination with transcatheter arterial chemoembolization (TACE) in rabbit VX2 liver cancer model. Material and Methods: Rabbits with VX2 liver tumors were randomized into four groups, eight in each group. After laparotomy and insertion of a 30-gauge needle into the proper hepatic artery, the following interventional procedure protocols were applied: 0.9% saline solution (group A, control), TACE (group B, TACE alone, lipiodol + mitomycin), intra-arterial interleukin-12 gene infusion (group C, IL-12 alone), and intra-arterial interleukin-12 gene infusion in combination with TACE (group D, IL-12 plus TACE). Growth ratio was estimated by computed tomography. To analyze apoptotic index, tumor tissues were explanted for terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining, 14 days after therapy. Results: Significant differences of the relative tumor growth ratio were observed in TACE alone group and IL-12 plus TACE group in comparison with control (P <0.05, ANOVA, Tukey's HSD correction) but not between IL-12 alone and control, or IL-12 plus TACE group and TACE alone group (P > 0.05). Significant changes of the apoptotic index were observed in group D in comparison with remaining three groups (P <0.05). The difference between group C and group A was not significant statistically (P > 0.05). Conclusion: Intra-arterial interleukin-12 gene therapy combined with TACE has a potent anti-tumor effect in rabbit VX2 liver cancer in comparison with TACE alone.

AB - Background: Interleukin-12 (IL-12), a cytokine naturally secreted by activated dendritic cells and monocytes/macrophages, is known as a key anti-tumor agent in many tumor models, including hepatocellular carcinoma (HCC) models. Purpose: To evaluate the anti-tumor effect of intra-arterial IL-12 gene delivery alone and in combination with transcatheter arterial chemoembolization (TACE) in rabbit VX2 liver cancer model. Material and Methods: Rabbits with VX2 liver tumors were randomized into four groups, eight in each group. After laparotomy and insertion of a 30-gauge needle into the proper hepatic artery, the following interventional procedure protocols were applied: 0.9% saline solution (group A, control), TACE (group B, TACE alone, lipiodol + mitomycin), intra-arterial interleukin-12 gene infusion (group C, IL-12 alone), and intra-arterial interleukin-12 gene infusion in combination with TACE (group D, IL-12 plus TACE). Growth ratio was estimated by computed tomography. To analyze apoptotic index, tumor tissues were explanted for terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining, 14 days after therapy. Results: Significant differences of the relative tumor growth ratio were observed in TACE alone group and IL-12 plus TACE group in comparison with control (P <0.05, ANOVA, Tukey's HSD correction) but not between IL-12 alone and control, or IL-12 plus TACE group and TACE alone group (P > 0.05). Significant changes of the apoptotic index were observed in group D in comparison with remaining three groups (P <0.05). The difference between group C and group A was not significant statistically (P > 0.05). Conclusion: Intra-arterial interleukin-12 gene therapy combined with TACE has a potent anti-tumor effect in rabbit VX2 liver cancer in comparison with TACE alone.

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KW - Apoptotic index

KW - Chemoembolization

KW - Gene therapy

KW - Interleukin-12

KW - Liver cancer

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