Poly(A) RNA was prepared from the intestine of anglerfish and was translated in a wheat germ cell-free system supplemented with 35S-methionine. SDS polyacrylamide gel electrophoresis of the labeled translation products revealed that the intestinal poly(A) RNA directs the synthesis of many proteins. Immunoprecipitations of the intestinal cell-free translation products with an antiserum to glucagon known to recognize anglerfish islet pre-proglucagon failed to identify an intestinal glucagon precursor. However, sensitive techniques of hybridization with a 32P-labelled cDNA containing the coding sequence for pancreatic glucagon identified a complementary RNA in the intestine. The mRNA of 620 bases is similar in size to the pre-proglucagon RNA in the islets (620-650 bases). These observations indicate that a gene encoding glucagon is expressed in the intestine, and that the mRNA encoding the intestinal glucagon precursor is of similar size to the pre-proglucagon mRNAs identified in the islets.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 30 1981|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology