Intestinal glucagon mRNA identified by hybridization to a cloned islet cDNA encoding a precursor

P. Kay Lund, Richard H. Goodman, Joel F. Habener

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Poly(A) RNA was prepared from the intestine of anglerfish and was translated in a wheat germ cell-free system supplemented with 35S-methionine. SDS polyacrylamide gel electrophoresis of the labeled translation products revealed that the intestinal poly(A) RNA directs the synthesis of many proteins. Immunoprecipitations of the intestinal cell-free translation products with an antiserum to glucagon known to recognize anglerfish islet pre-proglucagon failed to identify an intestinal glucagon precursor. However, sensitive techniques of hybridization with a 32P-labelled cDNA containing the coding sequence for pancreatic glucagon identified a complementary RNA in the intestine. The mRNA of 620 bases is similar in size to the pre-proglucagon RNA in the islets (620-650 bases). These observations indicate that a gene encoding glucagon is expressed in the intestine, and that the mRNA encoding the intestinal glucagon precursor is of similar size to the pre-proglucagon mRNAs identified in the islets.

Original languageEnglish (US)
Pages (from-to)1659-1666
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume100
Issue number4
DOIs
StatePublished - Jun 30 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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