Interleukin-2 secretion and transmembrane signalling in burned patients

Julita A. Teodorczyk-Injeyan, B. G. Sparkes, R. E. Falk, W. J. Peters, G. B. Mills

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

IL2 secretion in response to the T-cell mitogen Staphylococcal protein A (SPA) is significantly decreased in patients with major burns (n = 10, > 20% total body surface area) up to 50 days postburn in comparison with normal control (> 2 to 10 U/ml and 16 to 36 U/ml, respectively). Activation of protein kinase C (PK-C) and changes in [Ca++]i are both normally implicated in the production of IL2. Bypassing the requirements for mitogen-induced increases in [Ca++]i, using the cation ionophore A23187, or activating PK-C with the phorbol ester 12-o-tetradecanoyl-phorbol-13-acetate (TPA), failed to significantly restore SPA-induced IL2 production in cell cultures from burned patients. The combination of A23187 and TPA significantly (p < 0.005-0.001) enhanced IL2 secretion in patients’ cell cultures (range, 20 to > 64 U/ml). However, the levels of IL2 from the burned patients’ cultures remained significantly lower (p < 0.05) than those in control cultures exposed to TPA and A23187 (range, 256 to > 600 U/ml). Therefore, the burn-related defect in mitogen-induced IL2 secretion is only partially bypassed with cation ionophores and phorbol esters. This suggests that the abnormality in IL2 production may not be solely related to changes in PK-C activation and in [Ca++]i but may reside in other than transmembrane signalling mechanisms required for IL2 production.

Original languageEnglish (US)
Pages (from-to)152-157
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume28
Issue number2
DOIs
StatePublished - Feb 1988
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

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