Interleukin-2 secretion and transmembrane signalling in burned patients

Julita A. Teodorczyk-Injeyan, B. G. Sparkes, R. E. Falk, W. J. Peters, Gordon Mills

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

IL2 secretion in response to the T-cell mitogen Staphylococcal protein A (SPA) is significantly decreased in patients with major burns (n = 10, > 20% total body surface area) up to 50 days postburn in comparison with normal control (> 2 to 10 U/ml and 16 to 36 U/ml, respectively). Activation of protein kinase C (PK-C) and changes in [Ca++]i are both normally implicated in the production of IL2. Bypassing the requirements for mitogen-induced increases in [Ca++]i, using the cation ionophore A23187, or activating PK-C with the phorbol ester 12-o-tetradecanoyl-phorbol-13-acetate (TPA), failed to significantly restore SPA-induced IL2 production in cell cultures from burned patients. The combination of A23187 and TPA significantly (p < 0.005-0.001) enhanced IL2 secretion in patients’ cell cultures (range, 20 to > 64 U/ml). However, the levels of IL2 from the burned patients’ cultures remained significantly lower (p < 0.05) than those in control cultures exposed to TPA and A23187 (range, 256 to > 600 U/ml). Therefore, the burn-related defect in mitogen-induced IL2 secretion is only partially bypassed with cation ionophores and phorbol esters. This suggests that the abnormality in IL2 production may not be solely related to changes in PK-C activation and in [Ca++]i but may reside in other than transmembrane signalling mechanisms required for IL2 production.

Original languageEnglish (US)
Pages (from-to)152-157
Number of pages6
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume28
Issue number2
StatePublished - Jan 1 1988
Externally publishedYes

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Interleukin-2
Mitogens
Protein Kinase C
Ionophores
Calcimycin
Staphylococcal Protein A
Phorbol Esters
Burns
Cations
Acetates
Body Surface Area
Cell Culture Techniques
T-Lymphocytes

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Surgery

Cite this

Teodorczyk-Injeyan, J. A., Sparkes, B. G., Falk, R. E., Peters, W. J., & Mills, G. (1988). Interleukin-2 secretion and transmembrane signalling in burned patients. Journal of Trauma - Injury, Infection and Critical Care, 28(2), 152-157.

Interleukin-2 secretion and transmembrane signalling in burned patients. / Teodorczyk-Injeyan, Julita A.; Sparkes, B. G.; Falk, R. E.; Peters, W. J.; Mills, Gordon.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 28, No. 2, 01.01.1988, p. 152-157.

Research output: Contribution to journalArticle

Teodorczyk-Injeyan, JA, Sparkes, BG, Falk, RE, Peters, WJ & Mills, G 1988, 'Interleukin-2 secretion and transmembrane signalling in burned patients', Journal of Trauma - Injury, Infection and Critical Care, vol. 28, no. 2, pp. 152-157.
Teodorczyk-Injeyan, Julita A. ; Sparkes, B. G. ; Falk, R. E. ; Peters, W. J. ; Mills, Gordon. / Interleukin-2 secretion and transmembrane signalling in burned patients. In: Journal of Trauma - Injury, Infection and Critical Care. 1988 ; Vol. 28, No. 2. pp. 152-157.
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