Interleukin 2-induced tyrosine phosphorylation. Interleukin 2 receptor β is tyrosine phosphorylated

G. B. Mills, C. May, M. McGill, M. Fung, M. Baker, R. Sutherland, W. C. Greene

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    127 Scopus citations

    Abstract

    Interaction of interleukin 2 (IL2) with its high affinity membrane receptor complex (IL2R) is sufficient to induce proliferation of T lymphocytes. However, the biochemical mechanisms by which IL2 induces this process remain unresolved. The IL2R complex consists of at least two distinct polypeptides that bind IL2, a 75-kDa intermediate affinity subunit (IL2Rβ) and a 55-kDa low affinity subunit (IL2Rα). As indicated by Western blotting with anti-phosphotyrosine-specific antibodies and confirmed by phosphoamino acid analysis, we now demonstrate that interaction of the T cell growth factor interleukin 2 (IL2) with its high affinity receptor on IL2-sensitive human peripheral blood lymphoblasts induces tyrosine phosphorylation of proteins of 92, 80, 78, 70-75, and 57 kDa. IL2 induced tyrosine phosphorylation in YT 2C2 cells which express only the 75-kDa intermediate affinity IL2 binding molecule (IL2Rβ) but not in cells which either express only the 55-kDa low affinity IL2 receptor molecule (IL2Rα) or no IL2-binding sites. Therefore, IL2Rβ, in the absence of IL2Rα, appears sufficient to transduce the transmembrane signal leading to tyrosine phosphorylation. Two different antibodies reactive with phosphotyrosine specifically immunoprecipitated IL2Rβ cross-linked to radiolabeled IL2. These findings suggest that IL2Rβ is a substrate for the tyrosine kinase which is activated by IL2 binding to its receptor. Thus, like several other growth factor receptors, activation of the IL2R results in an increase in tyrosine phosphorylation with the receptor itself serving as one substrate.

    Original languageEnglish (US)
    Pages (from-to)3561-3567
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume265
    Issue number6
    StatePublished - Mar 23 1990

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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  • Cite this

    Mills, G. B., May, C., McGill, M., Fung, M., Baker, M., Sutherland, R., & Greene, W. C. (1990). Interleukin 2-induced tyrosine phosphorylation. Interleukin 2 receptor β is tyrosine phosphorylated. Journal of Biological Chemistry, 265(6), 3561-3567.