Interleukin 2 does not induce phosphatidylinositol hydrolysis in activated T cells

G. B. Mills, D. J. Stewart, A. Mellors, E. W. Gelfand

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    74 Scopus citations

    Abstract

    Hydrolysis of phosphatidylinositol-4,5-bisphosphate to diacylglycerol and myoinositol-1,4,5-trisphosphate is thought to be a primary event in the activation of cells by some growth factors, mitogenic lectins, and oncogenes. The mechanism whereby interleukin 2 (IL 2) binding to its receptor on activated T lymphocytes leads to cell proliferation has not been determined. Because the mitogenic action of IL 2 resembles that of some growth factors, the possible role of phosphatidylinositol breakdown in the activation of T cells by IL 2 was examined. In human or murine IL 2-sensitive cells, incubation with IL 2 did not alter the rate of turnover of phosphatidylinositol, phosphatidylinositol-5-phosphate, phosphatidylinositol-4,5-bisphosphate, or phosphatidylcholine in 32PO4-loaded cells. IL 2 also did not alter either the isotopic labeling of diacylglycerol or [3H]arachidonic acid release from cells. In addition, IL 2 did not alter the rate of formation of the phosphatidylinositol breakdown products of myoinositol-1, 4, 5-trisphosphate, myoinositol-1,4-bisphosphate, or myoinositol-1-phosphate. In contrast, under similar conditions, IL 2 induced significant increases in [3H]thymidine incorporation and cell proliferation. Mitogenic lectins such as concanavalin A and phytohemagglutinin gave significant changes in isotopic labeling of phosphoinositols, diacylglycerols, and phosphatidylinositols, indicating that phosphatidylinositol hydrolysis induced by mitogenic lectins was detectable in the assay systems. IL 2, in contrast to other growth factors, does not appear to signal cells by increasing phosphatidylinositol breakdown.

    Original languageEnglish (US)
    Pages (from-to)3019-3024
    Number of pages6
    JournalJournal of Immunology
    Volume136
    Issue number8
    StatePublished - Jan 1 1986

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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    Mills, G. B., Stewart, D. J., Mellors, A., & Gelfand, E. W. (1986). Interleukin 2 does not induce phosphatidylinositol hydrolysis in activated T cells. Journal of Immunology, 136(8), 3019-3024.