Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells

G. C. Bagby, C. A. Dinarello, P. Wallace, C. Wagner, Steven Hefeneider, E. McCall

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Studies designed to characterize monocyte-derived recruiting activity (MRA) a monokine that stimulates endothelial cells to produce granulocyte macrophage-colony-stimulating activity (CSA) by endothelial cells, show that it is a thermolabile protein of from 12,000 to 24,000 D which, on chromatofocusing, shows three separate peaks of eluted activity from pH 7.5 to 5.0. Because these and many other properties of MRA are identical to those of interleukin 1 (IL-1), we tested the hypothesis that MRA and IL-1 are identical. We cultured vascular endothelial cells with various concentrations of purified native and recombinant IL-1 (pI 7 form), then tested the endothelial cell supernatants for GM-CSA. Purified native IL-1 and recombinant IL-1 stimulated endothelial cells to release CSA. The MRA of native IL-1, recombinant IL-1, and unfractionated monocyte conditioned medium was neutralized by a highly specific rabbit anti-human IL-1 antiserum. Chromatofocusing fractions that contained MRA contained immunoreacive IL-1 on immunoblotting and the bioactivity was neutralized completely by treatment with the antiserum. We conclude that IL-1 induces the release of CSA by vascular endothelial cells, that IL-1 is constitutively produced by monocytes in vitro, and that MRA and IL-1 are biologically, biophysically and, immunologically identical.

Original languageEnglish (US)
Pages (from-to)1316-1323
Number of pages8
JournalJournal of Clinical Investigation
Volume78
Issue number5
StatePublished - 1986
Externally publishedYes

Fingerprint

Interleukin-1
Granulocytes
Endothelial Cells
Macrophages
Monocytes
Immune Sera
Monokines
Conditioned Culture Medium
Immunoblotting
Rabbits

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells. / Bagby, G. C.; Dinarello, C. A.; Wallace, P.; Wagner, C.; Hefeneider, Steven; McCall, E.

In: Journal of Clinical Investigation, Vol. 78, No. 5, 1986, p. 1316-1323.

Research output: Contribution to journalArticle

Bagby, G. C. ; Dinarello, C. A. ; Wallace, P. ; Wagner, C. ; Hefeneider, Steven ; McCall, E. / Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells. In: Journal of Clinical Investigation. 1986 ; Vol. 78, No. 5. pp. 1316-1323.
@article{39a472e0a19144d4adef3369eed3b261,
title = "Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells",
abstract = "Studies designed to characterize monocyte-derived recruiting activity (MRA) a monokine that stimulates endothelial cells to produce granulocyte macrophage-colony-stimulating activity (CSA) by endothelial cells, show that it is a thermolabile protein of from 12,000 to 24,000 D which, on chromatofocusing, shows three separate peaks of eluted activity from pH 7.5 to 5.0. Because these and many other properties of MRA are identical to those of interleukin 1 (IL-1), we tested the hypothesis that MRA and IL-1 are identical. We cultured vascular endothelial cells with various concentrations of purified native and recombinant IL-1 (pI 7 form), then tested the endothelial cell supernatants for GM-CSA. Purified native IL-1 and recombinant IL-1 stimulated endothelial cells to release CSA. The MRA of native IL-1, recombinant IL-1, and unfractionated monocyte conditioned medium was neutralized by a highly specific rabbit anti-human IL-1 antiserum. Chromatofocusing fractions that contained MRA contained immunoreacive IL-1 on immunoblotting and the bioactivity was neutralized completely by treatment with the antiserum. We conclude that IL-1 induces the release of CSA by vascular endothelial cells, that IL-1 is constitutively produced by monocytes in vitro, and that MRA and IL-1 are biologically, biophysically and, immunologically identical.",
author = "Bagby, {G. C.} and Dinarello, {C. A.} and P. Wallace and C. Wagner and Steven Hefeneider and E. McCall",
year = "1986",
language = "English (US)",
volume = "78",
pages = "1316--1323",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "5",

}

TY - JOUR

T1 - Interleukin 1 stimulates granulocyte macrophage colony-stimulating activity release by vascular endothelial cells

AU - Bagby, G. C.

AU - Dinarello, C. A.

AU - Wallace, P.

AU - Wagner, C.

AU - Hefeneider, Steven

AU - McCall, E.

PY - 1986

Y1 - 1986

N2 - Studies designed to characterize monocyte-derived recruiting activity (MRA) a monokine that stimulates endothelial cells to produce granulocyte macrophage-colony-stimulating activity (CSA) by endothelial cells, show that it is a thermolabile protein of from 12,000 to 24,000 D which, on chromatofocusing, shows three separate peaks of eluted activity from pH 7.5 to 5.0. Because these and many other properties of MRA are identical to those of interleukin 1 (IL-1), we tested the hypothesis that MRA and IL-1 are identical. We cultured vascular endothelial cells with various concentrations of purified native and recombinant IL-1 (pI 7 form), then tested the endothelial cell supernatants for GM-CSA. Purified native IL-1 and recombinant IL-1 stimulated endothelial cells to release CSA. The MRA of native IL-1, recombinant IL-1, and unfractionated monocyte conditioned medium was neutralized by a highly specific rabbit anti-human IL-1 antiserum. Chromatofocusing fractions that contained MRA contained immunoreacive IL-1 on immunoblotting and the bioactivity was neutralized completely by treatment with the antiserum. We conclude that IL-1 induces the release of CSA by vascular endothelial cells, that IL-1 is constitutively produced by monocytes in vitro, and that MRA and IL-1 are biologically, biophysically and, immunologically identical.

AB - Studies designed to characterize monocyte-derived recruiting activity (MRA) a monokine that stimulates endothelial cells to produce granulocyte macrophage-colony-stimulating activity (CSA) by endothelial cells, show that it is a thermolabile protein of from 12,000 to 24,000 D which, on chromatofocusing, shows three separate peaks of eluted activity from pH 7.5 to 5.0. Because these and many other properties of MRA are identical to those of interleukin 1 (IL-1), we tested the hypothesis that MRA and IL-1 are identical. We cultured vascular endothelial cells with various concentrations of purified native and recombinant IL-1 (pI 7 form), then tested the endothelial cell supernatants for GM-CSA. Purified native IL-1 and recombinant IL-1 stimulated endothelial cells to release CSA. The MRA of native IL-1, recombinant IL-1, and unfractionated monocyte conditioned medium was neutralized by a highly specific rabbit anti-human IL-1 antiserum. Chromatofocusing fractions that contained MRA contained immunoreacive IL-1 on immunoblotting and the bioactivity was neutralized completely by treatment with the antiserum. We conclude that IL-1 induces the release of CSA by vascular endothelial cells, that IL-1 is constitutively produced by monocytes in vitro, and that MRA and IL-1 are biologically, biophysically and, immunologically identical.

UR - http://www.scopus.com/inward/record.url?scp=0023026845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023026845&partnerID=8YFLogxK

M3 - Article

C2 - 3490494

AN - SCOPUS:0023026845

VL - 78

SP - 1316

EP - 1323

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 5

ER -