TY - JOUR
T1 - Interleukin 1 stimulates endothelial cells to release multilineage human colony-stimulating activity
AU - Segal, G. M.
AU - McCall, E.
AU - Stueve, T.
AU - Bagby, G. C.
PY - 1987
Y1 - 1987
N2 - Cultured human umbilical vein endothelial cells, when exposed to soluble products of peripheral blood monocytes, elaborate granulocyte-macrophage colony-stimulating activity (GM-CSA) and erythroid burst-promoting activity (BPA). We have performed studies to determine if the monokine IL 1 can stimulate endothelial cells to release hematopoietic growth factors and whether such factors can also support human megakaryocyte (Meg) and mixed-cell colony growth. Various concentrations of recombinant human IL 1β (rIL 1) and media conditioned by monocytes (MCM), endothelial cells (ECM), and endothelial cells cultured for 3 days in 50% MCM (ECM(M)) or rIL 1 (ECM(rIL 1)) were added to marrow mononuclear cells cultured in methylcellulose. ECM(M) and ECM(rIL 1) stimulated, in a dose-dependent fashion, the growth of Meg, mixed-cell, and GM colonies and erythroid bursts. In contrast, ECM, MCM, and rIL 1 displayed little or no activity in the colony-forming assays. Preincubation with specific antisera to native human IL 1 or rIL 1 reduced by 75 to 100% the activity of MCM in stimulating endothelial cell release of BPA, GM-CSA, Meg-CSA, and mixed-cell CSA. Meg-CSA, although readily detectable at ECM(M) and ECM(rIL 1) concentrations in culture of 1 to 5%, was partially masked by lineage-specific inhibitors of Meg colony growth. When ECM(M) was analyzed by gel filtration chromatography, the megakaryocytopoietic inhibitory activity eluted in the high M(r) fractions (>75 kD). Meg-CSA co-eluted with GM-CSA and BPA in a single peak of 30 kD. We conclude that endothelial cells, in response to IL 1, produce one or more growth factors that probably act on multiple classes of progenitor cells.
AB - Cultured human umbilical vein endothelial cells, when exposed to soluble products of peripheral blood monocytes, elaborate granulocyte-macrophage colony-stimulating activity (GM-CSA) and erythroid burst-promoting activity (BPA). We have performed studies to determine if the monokine IL 1 can stimulate endothelial cells to release hematopoietic growth factors and whether such factors can also support human megakaryocyte (Meg) and mixed-cell colony growth. Various concentrations of recombinant human IL 1β (rIL 1) and media conditioned by monocytes (MCM), endothelial cells (ECM), and endothelial cells cultured for 3 days in 50% MCM (ECM(M)) or rIL 1 (ECM(rIL 1)) were added to marrow mononuclear cells cultured in methylcellulose. ECM(M) and ECM(rIL 1) stimulated, in a dose-dependent fashion, the growth of Meg, mixed-cell, and GM colonies and erythroid bursts. In contrast, ECM, MCM, and rIL 1 displayed little or no activity in the colony-forming assays. Preincubation with specific antisera to native human IL 1 or rIL 1 reduced by 75 to 100% the activity of MCM in stimulating endothelial cell release of BPA, GM-CSA, Meg-CSA, and mixed-cell CSA. Meg-CSA, although readily detectable at ECM(M) and ECM(rIL 1) concentrations in culture of 1 to 5%, was partially masked by lineage-specific inhibitors of Meg colony growth. When ECM(M) was analyzed by gel filtration chromatography, the megakaryocytopoietic inhibitory activity eluted in the high M(r) fractions (>75 kD). Meg-CSA co-eluted with GM-CSA and BPA in a single peak of 30 kD. We conclude that endothelial cells, in response to IL 1, produce one or more growth factors that probably act on multiple classes of progenitor cells.
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M3 - Article
C2 - 3493286
AN - SCOPUS:0023134026
SN - 0022-1767
VL - 138
SP - 1772
EP - 1778
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -